Human LEF1 Antibody

Catalog #: MAB7647 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7647
MAB7647-SP

Detection of LEF1 in Human Spleen Tissue.

1 Image

All LEF1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LEF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LEF1 antibody in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1071412

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

LEF1 containing synthetic peptide
Accession # Q9UJU2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5 µg/mL

Human Spleen Tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Detection of LEF1 in Human Spleen Tissue. LEF1 was detected in Formalin Fixed fixed Paraffin Embedded sections of human spleen tissue using Mouse Anti-Human LEF1 Monoclonal Antibody (Catalog # MAB7647) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LEF1

LEF1 (Lymphoid Enhancer-binding Factor 1) is a functionally diverse member of the TCF/LEF family of transcription factors. The name LEF1 was originally applied to the mouse factor, while its human counterpart was named TCF-1 alpha. Since there is a related molecule named TCF-1, to avoid confusion with TCF-1 alpha, both species factors are now called LEF1. Although its predicted MW is 44 kDa, it runs anomalously at 35-55 kDa in SDS-PAGE. LEF1 has restricted expression in adult, being limited to T cells, thymocytes, pre-B cells and NK cells. LEF1 demonstrates sequence-specific (CCTTTG[T/A][T/A]) DNA binding and directs beta -catenin to Wnt‑responsive genes. Either transcriptional activation or repression may occur on LEF1 target genes, depending upon 1) the cofactors recruited to the LEF1: beta ‑catenin complex, and 2) the phosphorylation state of LEF1. Human LEF1 is 399 amino acids (aa) in length. It contains one DNA-binding HMG-box (aa 298‑369), three potential phosphorylation sites, and an N-terminal SUMOylation site at Lys27. There are at least five isoform variants. One utilizes an alternative start site at Met116, a second contains a 47 aa substitution for aa 283‑299, and a third combines the previous two variations. A fourth variant shows a deletion of aa 214‑241, while a fifth variant combines the afore mentioned deletion of aa 214-241 with a 25 aa substitution for aa 390‑399. Over aa 120‑280, human LEF1 shares 97% aa sequence identity with mouse LEF1.

Long Name

Lymphoid Enhancer-binding Factor 1

Entrez Gene IDs

51176 (Human); 16842 (Mouse); 161452 (Rat)

Alternate Names

DKFZp586H0919; FLJ46390; LEF1; LEF-1; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF10; TCF1ALPHA; TCF1-alpha; TCF7L3