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Human LIF Antibody

Catalog #: AF-250-NA
17 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-250-NA
AF-250-SP
LIF in Human Lung.
3 Images
Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LIF Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIF in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LIF
Ser23-Phe202
Accession # P15018
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04-0.08 µg/mL in the presence of 3 ng/mL Recombinant Human LIF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry LIF antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

LIF in Human Lung. LIF was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry LIF antibody in Human Alzheimer's Brain by Immunohistochemistry (IHC-P). View Larger

LIF in Human Alzheimer's Brain. LIF was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. View Larger

Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF (7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-depend-ent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA). The ND50 is typically 0.04-0.08 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

LIF (Leukemia inhibitory factor; also  Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of  the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.

Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

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Citations for Human LIF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Leukemia inhibitory factor drives glucose metabolic reprogramming to promote breast tumorigenesis
    Authors: X Yue, J Wang, CY Chang, J Liu, X Yang, F Zhou, X Qiu, V Bhatt, JY Guo, X Su, L Zhang, Z Feng, W Hu
    Cell Death & Disease, 2022-04-19;13(4):370.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation
    Authors: M Kessler, K Hoffmann, K Fritsche, V Brinkmann, HJ Mollenkopf, O Thieck, AR Teixeira d, EI Braicu, J Sehouli, M Mangler, H Berger, TF Meyer
    Nat Commun, 2019-03-18;10(1):1194.
    Species: Human
    Sample Types: Organoids
    Applications: Neutralization
  3. Senescent fibroblasts drive ageing pigmentation: ?A potential therapeutic target for senile lentigo
    Authors: JE Yoon, Y Kim, S Kwon, M Kim, YH Kim, JH Kim, TJ Park, HY Kang
    Theranostics, 2018-09-09;8(17):4620-4632.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Decreased MUC1 in endometrium is an independent receptivity marker in recurrent implantation failure during implantation window
    Authors: F Wu, X Chen, Y Liu, B Liang, H Xu, TC Li, CC Wang
    Reprod. Biol. Endocrinol., 2018-06-21;16(1):60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Possible involvement of human mast cells in the establishment of pregnancy via KIR2DL4
    Authors: C Ueshima, TR Kataoka, M Hirata, A Sugimoto, Y Iemura, S Minamiguch, T Nomura, H Haga
    Am. J. Pathol., 2018-03-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Leukemia Inhibitory Factor (LIF) Inhibition during Mid-Gestation Impairs Trophoblast Invasion and Spiral Artery Remodelling during Pregnancy in Mice
    Authors: Amy Winship, Jeanne Correia, Jian-Guo Zhang, Nicos A. Nicola, Evdokia Dimitriadis
    PLOS ONE
  7. Blocking Endogenous Leukemia Inhibitory Factor During Placental Development in Mice Leads to Abnormal Placentation and Pregnancy Loss
    Authors: Amy Winship, Jeanne Correia, Tara Krishnan, Ellen Menkhorst, Carly Cuman, Jian-Guo Zhang et al.
    Scientific Reports
  8. Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.
    Authors: Xiao Y, Sun X, Yang X, Zhang J, Xue Q, Cai B, Zhou Y
    Fertil. Steril., 2009-04-09;94(1):85-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry-Paraffin
  9. Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
    Authors: Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
    Fertil. Steril., 2008-08-05;91(6):2602-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Effect of levonorgestrel and mifepristone on endometrial receptivity markers in a three-dimensional human endometrial cell culture model.
    Authors: Meng CX, Andersson KL, Bentin-Ley U, Gemzell-Danielsson K, Lalitkumar PG
    Fertil. Steril., 2008-01-18;91(1):256-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression.
    Authors: Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H
    Int. J. Oncol., 2007-04-01;30(4):977-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
    Authors: Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O
    Fertil. Steril., 2006-09-01;86(4):1193-209.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005-08-15;579(20):4317-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
    Authors: Andersson By U, Tani E, Andersson U
    J. Pediatr. Hematol. Oncol., 2004-11-01;26(11):706-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003-12-21;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
    Authors: Ben-Shlomo A, Miklovsky I, Ren SG, Yong WH, Heaney AP, Culler MD, Melmed S
    J. Clin. Endocrinol. Metab., 2003-02-01;88(2):858-63.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Leukemia inhibitory factor functions in parallel with interleukin-6 to promote ovarian cancer growth
    Authors: K McLean, L Tan, DE Bolland, LG Coffman, LF Peterson, M Talpaz, N Neamati, RJ Buckanovic
    Oncogene, 2018-10-10;0(0):.

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Human LIF Antibody
By Anonymous on 06/19/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skin tissue Species: Human
Immunocytochemistry/Immunofluorescence Human LIF Antibody AF-250-NA