Human LILRB5/CD85c/LIR-8 Antibody

Catalog #: MAB30651 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30651-100
MAB30651-SP

LILRB5/CD85c/LIR-8 in Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB5/CD85c/LIR-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB5/CD87c/LIR-8 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1058033

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human LILRB5/CD85c/LIR-8
Arg18-His456
Accession # O75023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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LILRB5/CD85c/LIR-8 in Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line. LILRB5/CD85c/LIR-8 was detected in immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-Human LILRB5/CD85c/LIR-8 Monoclonal Antibody (Catalog # MAB30651) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

LIR8, also known as CD85c and LILRB5, belongs to the Leukocyte immunoglobulin-like receptors (LILR) family of transmembrane glycoproteins involved in regulating immune responses (1,2). There are at least thirteen LILR family members and are divided into activating (LILRA) or inhibiting (LILRB) molecules (1, 2). Mature human LIR8 consists of an extracellular domain (ECD) with four Ig-like domains, a transmembrane segment, and a cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). Alternative splicing of human LIR8 generates at least 2 isoforms, one lacking the second Ig-like domain. The LILR family appears to be primate-specific receptors in terms of sequence homology. LIR8 is expressed on NK cells and in the tryptic granules of mast cells and negatively regulates immune cell activation (3, 4) It is present on the mast cell surface following cell activation and degranulation (4). Activated mast cells may also release soluble forms of LIR8 (3). LIR8 has also been shown to be expressed on T cells and induce CD8+ T cell proliferation (5). Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (6), R&D Systems in-house testing indicates that LIR8 binds to Angiopoietin-like 7. Recently, a common missense variant of LIR8 was found to be associated with statin intolerance and myalgia (7).

References

Brown, D. et al. (2004) Tissue antigens. 64:215.
Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
Borges, L. et al. (1997) J. Immunol. 159:5192.
Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
Hogan, H. E. et al. (2016) Sci Rep. 6:21780.
Zheng, J. et al. (2012) Nature 485:656.
Siddigui, M. et al. (2017) Eur. Heart J. 38:3569.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Alternate Names

CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5