Human LILRB5/CD85c/LIR-8 Antibody

Catalog #: MAB3065 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3065
MAB3065-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB5/CD85c/LIR-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, 2, 3, 4, 5, rhLIR-6, or rhILT11/LILRA5 is observed. In Western blots, less than 5% cross-reactivity with rhILT2, 3, 5, rhLIR-6 or rhILT11/LILRA5 is observed, and no cross-reactivity with rhILT1, 4, or 6.

Source

Monoclonal Mouse IgG_2B Clone # 395239

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8 isoform 1
Arg18-His456
Accession # O75023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human LILRB5/CD85c/LIR-8

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

Human LIR-8 (leukocyte immunoglobulin-like receptor 8; also CD85c and LILRB5) is a 567 amino acid (aa) member of the LIR family of receptors. It is a type I transmembrane protein that is expressed on NK cells. LIR-8 has a 435 aa extracellular domain with four C2-type Ig-like domains (aa 24‑458), and a 111 aa cytoplasmic tail that contains two ITIM motifs. Phosphorylation of Tyr in an ITIM motif recruits SHP-1, which is subsequently activated and impacts related signaling pathways. The ligand for, and function of, LIR-8 are unreported. LIR-8 has two potential splice forms. One shows a 53 aa cytoplasmic insert between Arg542 and Ala543. The second shows a deletion of 100 aa between Gly119 and Gly219 that impacts the second C2-type Ig-like domain. There is no rodent ortholog.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Alternate Names

CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5