Human LRRK2 Antibody

Catalog #: AF6674 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6674
AF6674-SP

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Human LRRK2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRRK2 in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LRRK2
Val241-Val500
Accession # Q5S007

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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LRRK2 in Human Brain. LRRK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human LRRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6674) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm and processes of neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRK2

LRRK2 (Leu-rich repeat/LRR-containing protein kinase 2; also Dardarin) is a 260-280 kDa member of the TKL (Tyr kinase-like) Ser/Thr protein kinase family of molecules. It is unusual in that it possesses both GTPase and protein kinase enzyme activity. LRRK2 is expressed in dopamine system neurons, macrophages and B2 B cells (in rodent), and mutations of the molecule are associated with the pathogenesis of Parkinson's disease. Through its protein kinase activity, LRRK2 phosphorylates both 4E-BP and FoxO1, two molecules involved in the initiation of protein translation. Human LRRK2 is 2527 amino acids (aa) in length. It contains multiple domains, including 16 LRRs (aa 226-249; 791-1291; 1556-1887), one coiled-coil region (aa 319-348), a GTPase Roc domain (aa 1328-1511), a protein kinase region (aa 1879-2138), and a WD40 domain (aa 2168-2387). There are three potential splice variants for LRRK2. One shows an alternative start site at Met1203 coupled to a 26 aa substitution for aa 2465-2527, a second contains a three aa substitution for aa 1-1165, and a third possesses an alternative start site at Met402 accompanied by a 12 aa substitution for aa 1259-2527. Over aa 241-500, human LRRK2 shares 85% aa identity with mouse LRRK2.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Entrez Gene IDs

120892 (Human); 66725 (Mouse); 300160 (Rat)

Alternate Names

augmented in rheumatoid arthritis 17; AURA17; Dardarin; DKFZp434H2111; EC 2.7.11.1; FLJ45829; leucine-rich repeat kinase 2; leucine-rich repeat serine/threonine-protein kinase 2; LRRK2; PARK8; PARK8DARDARIN; Parkinson disease (autosomal dominant) 8; RIPK7; ROCO2