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Human MMR/CD206 Antibody

Catalog #: AF2534
39 Citations 1 Review Datasheet / COA / SDS
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AF2534
AF2534-SP
Detection of Human MMR/CD206 by Western Blot.
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Citations (39)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMR/CD206 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MMR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMR/CD206
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
Human immature dendritic cells
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of human liver
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MMR/CD206 antibody by Western Blot. View Larger

Detection of Human MMR/CD206 by Western Blot. Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry View Larger

MMR/CD206 in Human Liver. MMR/CD206 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western View Larger

Detection of Human MMR/CD206 by Simple WesternTM. Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.2 mg/mL. A specific band was detected for MMR/CD206 at approximately 201 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMR/CD206

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References
  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Long Name
Macrophage Mannose Receptor
Entrez Gene IDs
4360 (Human); 17533 (Mouse); 291327 (Rat)
Alternate Names
CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1

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Citations for Human MMR/CD206 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Adipose tissue–specific ablation of Ces1d causes metabolic dysregulation in mice
    Authors: Gang Li, Xin Li, Li Yang, Shuyue Wang, Yulin Dai, Baharan Fekry et al.
    Life Science Alliance
  2. Characterization of macrophage polarization in periodontal disease
    Authors: Carlos Garaicoa‐Pazmino, Tobias Fretwurst, Cristiane H. Squarize, Tord Berglundh, William V. Giannobile, Lena Larsson et al.
    Journal of Clinical Periodontology
  3. Immunohistochemical Identification of Human Skeletal Muscle Macrophages
    Authors: Kate Kosmac, Bailey D. Peck, R. Grace Walton, Jyothi Mula, Philip A. Kern, Marcas M. Bamman et al.
    BIO-PROTOCOL
  4. Important functional role of the protein osteopontin in the progression of malignant pleural mesothelioma
    Authors: Elisabeth Digifico, Marco Erreni, Laura Mannarino, Sergio Marchini, Aldo Ummarino, Clément Anfray et al.
    Frontiers in Immunology
  5. Opposing functions of ?-arrestin 1 and 2 in Parkinson's disease via microglia inflammation and Nprl3
    Authors: Fang Y, Jiang Q, Li S et al.
    Cell Death & Differentiation
  6. Age-dependent regulation of cell-mediated collagen turnover
    Authors: Michael J. Podolsky, Christopher D. Yang, Carlos Lizama Valenzuela, Ritwik Datta, Steven K. Huang, Stephen L. Nishimura et al.
    JCI Insight
  7. IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin
    Authors: Satoshi Horiba, Ryota Kami, Taiki Tsutsui, Junichi Hosoi
    JID Innovations
  8. Tumor-associated macrophages respond to chemotherapy by detrimental transcriptional reprogramming and suppressing stabilin-1 mediated clearance of EGF
    Authors: Larionova I, Kiselev A, Kazakova E et al.
    Frontiers in immunology
  9. Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity
    Authors: Dos Santos, JC;Moreno, M;Teufel, LU;Chilibroste, S;Keating, ST;Groh, L;Domínguez-Andrés, J;Williams, DL;Ma, Z;Lowman, DW;Ensley, HE;Novakovic, B;Ribeiro-Dias, F;Netea, MG;Chabalgoity, JA;Joosten, LAB;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  10. Graph Fourier transform for spatial omics representation and analyses of complex organs
    Authors: Ma, Q;Chang, Y;Liu, J;Jiang, Y;Ma, A;Yeo, YY;Guo, Q;McNutt, M;Krull, J;Rodig, S;Barouch, D;Nolan, G;Xu, D;Jiang, S;Li, Z;Liu, B;
    Research square
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosis
    Authors: Antwi, MB;Dumitriu, G;Simón-Santamaria, J;Romano, JS;Li, R;Smedsrød, B;Vik, A;Eskild, W;Sørensen, KK;
    PloS one
    Species: Transgenic Mouse, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  12. Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cells
    Authors: KW Southerlan, Y Xu, DT Peters, X Wei, X Lin, Y Xiang, K Fei, LA Olivere, JM Morowitz, J Otto, Q Dai, CD Kontos, Y Diao
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. CD206+ tumor-associated macrophages interact with CD4+ tumor-infiltrating lymphocytes and predict adverse patient outcome in human laryngeal squamous cell carcinoma
    Authors: Y Heng, X Zhu, H Lin, M Jingyu, X Ding, L Tao, L Lu
    Journal of Translational Medicine, 2023-03-03;21(1):167.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques
    Authors: P Madden, Y Thomas, R Blair, S Samer, M Doyle, C Midkiff, L Doyle-Meye, M Becker, S Arif, M McRaven, L Simons, A Carias, E Martinelli, R Lorenzo-Re, J Hultquist, F Villinger, R Veazey, T Hope
    Research square, 2022-04-08;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  15. Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
    Authors: BS Finlin, H Memetimin, AL Confides, B Zhu, PM Westgate, EE Dupont-Ver, PA Kern
    Scientific Reports, 2021-12-08;11(1):23598.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton's Jelly-A Comparative Study
    Authors: M Dymowska, A Aksamit, K Zielniok, M Kniotek, B Kaleta, A Roszczyk, M Zych, F Dabrowski, L Paczek, A Burdzinska
    Pharmaceutics, 2021-11-01;13(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Partial Ablation of Non-Myogenic Progenitor Cells as a Therapeutic Approach to Duchenne Muscular Dystrophy
    Authors: Z Gao, A Lu, AC Daquinag, Y Yu, M Huard, C Tseng, X Gao, J Huard, MG Kolonin
    Biomolecules, 2021-10-15;11(10):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Opposing functions of ?-arrestin 1 and 2 in Parkinson's disease via microglia inflammation and Nprl3
    Authors: Fang Y, Jiang Q, Li S et al.
    Cell Death & Differentiation
  19. The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix
    Authors: S Taylor, M Whitfield, J Barratt, A Didangelos
    J. Immunol., 2020-09-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
    Authors: K Kosmac, M Gonzalez-F, MM McDermott, SH White, RG Walton, RL Sufit, L Tian, L Li, MR Kibbe, MH Criqui, JM Guralnik, T S Polonsky, C Leeuwenbur, L Ferrucci, CA Peterson
    J Am Heart Assoc, 2020-05-09;9(10):e015929.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Skewed macrophage polarization in aging skeletal muscle
    Authors: CY Cui, RK Driscoll, Y Piao, CW Chia, M Gorospe, L Ferrucci
    Aging Cell, 2019-09-02;0(0):e13032.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Markers of adipose tissue inflammation are transiently elevated during intermittent fasting in women who are overweight or obese
    Authors: B Liu, AT Hutchison, CH Thompson, K Lange, LK Heilbronn
    Obes Res Clin Pract, 2019-07-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms
    Authors: MC Nielsen, MN Andersen, N Rittig, S Rødgaard-H, H Grønbaek, SK Moestrup, HJ Møller, A Etzerodt
    J. Leukoc. Biol., 2019-06-26;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  24. Interaction with tumor?associated macrophages promotes PRL?3?induced invasion of colorectal cancer cells via MAPK pathway?induced EMT and NF??B signaling?induced angiogenesis
    Authors: T Zhang, L Liu, W Lai, Y Zeng, H Xu, Q Lan, P Su, Z Chu
    Oncol. Rep., 2019-03-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. A glucose-responsive insulin therapy protects animals against hypoglycemia
    Authors: R Yang, M Wu, S Lin, RP Nargund, X Li, T Kelly, L Yan, G Dai, Y Qian, Q Dallas-Yan, PA Fischer, Y Cui, X Shen, P Huo, DD Feng, MD Erion, DE Kelley, J Mu
    JCI Insight, 2018-01-11;3(1):.
    Applications: Bioassay
  26. Regulatory role of cytosolic phospholipase A2 alpha in the induction of CD40 in microglia
    Authors: YF Malada-Ede, N Hadad, R Levy
    J Neuroinflammation, 2017-02-10;14(1):33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides
    PLoS ONE, 2016-08-05;11(8):e0160602.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease.
    Authors: Millar N, Gilchrist D, Akbar M, Reilly J, Kerr S, Campbell A, Murrell G, Liew F, Kurowska-Stolarska M, McInnes I
    Nat Commun, 2015-04-10;6(0):6774.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Pioglitazone treatment reduces adipose tissue inflammation through reduction of mast cell and macrophage number and by improving vascularity.
    Authors: Spencer M, Yang L, Adu A, Finlin B, Zhu B, Shipp L, Rasouli N, Peterson C, Kern P
    PLoS ONE, 2014-07-10;9(7):e102190.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. Targeting the ANG2/TIE2 axis inhibits tumor growth and metastasis by impairing angiogenesis and disabling rebounds of proangiogenic myeloid cells.
    Authors: Mazzieri R, Pucci F, Moi D, Zonari E, Ranghetti A, Berti A, Politi LS, Gentner B, Brown JL, Naldini L, De Palma M
    Cancer Cell, 2011-04-12;19(4):512-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Acid fibroblast growth factor and peripheral nerve grafts regulate Th2 cytokine expression, macrophage activation, polyamine synthesis, and neurotrophin expression in transected rat spinal cords.
    Authors: Kuo HS, Tsai MJ, Huang MC
    J. Neurosci., 2011-03-16;31(11):4137-47.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  32. The immune system in sporadic inclusion body myositis patients is not compromised by blood-flow restricted exercise training
    Authors: Kasper Yde Jensen, Mikkel Jacobsen, Henrik Daa Schrøder, Per Aagaard, Jakob Lindberg Nielsen, Anders Nørkær Jørgensen et al.
    Arthritis Research & Therapy
  33. Autofluorescence in freshly isolated adult human liver sinusoidal cells
    Authors: Anett Kristin Larsen, Jaione Simón-Santamaría, Kjetil Elvevold, Bo Göran Ericzon, Kim Erlend Mortensen, Peter McCourt et al.
    European Journal of Histochemistry
  34. The soluble mannose receptor (sMR/sCD206) in critically ill patients with invasive fungal infections, bacterial infections or non-infectious inflammation: a secondary analysis of the EPaNIC RCT
    Authors: Greet De Vlieger, Ilse Vanhorebeek, Pieter J. Wouters, Inge Derese, Michael P. Casaer, Yves Debaveye et al.
    Critical Care
  35. Skeletal muscle fibrosis is associated with decreased muscle inflammation and weakness in patients with chronic kidney disease
    Authors: Matthew K. Abramowitz, William Paredes, Kehao Zhang, Camille R. Brightwell, Julia N. Newsom, Hyok-Joon Kwon et al.
    American Journal of Physiology-Renal Physiology
  36. Efficient Uptake of Blood-Borne BK and JC Polyomavirus-Like Particles in Endothelial Cells of Liver Sinusoids and Renal Vasa Recta
    Authors: Jaione Simon-Santamaria, Christine Hanssen Rinaldo, Piotr Kardas, Ruomei Li, Ivana Malovic, Kjetil Elvevold et al.
    PLoS ONE
  37. Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages.
    Authors: Bhandari, S, Li, R Et al.
    BMC Mol Cell Biol
  38. Tissue Levels of CD80, CD163 and CD206 and Their Ratios in Periodontal and Peri-Implant Health and Disease
    Authors: Mustafa Yilmaz, Esra Demir, Yigit Firatli, Erhan Firatli, Ulvi Kahraman Gürsoy
    Current Issues in Molecular Biology
  39. PD-L1 CAR effector cells induce self-amplifying cytotoxic effects against target cells
    Authors: Malgorzata Bajor, Agnieszka Graczyk-Jarzynka, Katsiaryna Marhelava, Anna Burdzinska, Angelika Muchowicz, Agnieszka Goral et al.
    Journal for ImmunoTherapy of Cancer

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Human MMR/CD206 Antibody
By Anonymous on 09/23/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

I used this antibody for developing a sandwich ELISA in combination with mAb (cat.MAB25341) and protein (cat.2534-MR). This combination gives good standard curve but unfortunately did not detect any MMR in our samples.

ELISA Human MMR/CD206 Antibody AF2534