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Human/Mouse KLF5 Antibody

Catalog #: AF3758
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3758
AF3758-SP
Detection of Human and Mouse KLF5 by Western Blot.
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Product Details
Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse KLF5 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human KLF5
Leu85-Ala177
Accession # Q13887
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
KLF5 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in KLF5 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse KLF5 antibody by Western Blot. View Larger

Detection of Human and Mouse KLF5 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry KLF5 antibody in D3 Mouse Stem Cells by Immunocytochemistry (ICC). View Larger

KLF5 in D3 Mouse Stem Cells. KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse KLF5 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse KLF5 by Simple WesternTM. Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human KLF5 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human KLF5 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for KLF5 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF5

Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.

Long Name
Kruppel-Like Factor 5
Entrez Gene IDs
688 (Human)
Alternate Names
Basic transcription element-binding protein 2; BTEB2; BTE-binding protein 2; CKLFGC-box-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; GC box binding protein 2; IKLFbasic transcription element binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; KLF5; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); Transcription factor BTEB2

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Citations for Human/Mouse KLF5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic Pancreatitis
    Authors: Alavi, M;Mejia-Bautista, A;Tang, M;Bandovic, J;Rosenberg, AZ;Bialkowska, AB;
    Cancers
    Species: Mouse
    Sample Types: Whole Tissues
    Applications: IHC
  2. Targeting the KLF5-EphA2 axis can restrain cancer stemness and overcome chemoresistance in basal-like breast cancer
    Authors: P Zhao, J Sun, X Huang, X Zhang, X Liu, R Liu, G Du, W Gan, C Yang, Y Tang, C Chen, D Jiang
    International journal of biological sciences, 2023-03-21;19(6):1861-1874.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot, ChIP
  3. Histone Deacetylase Inhibitors (HDACi) Promote KLF5 Ubiquitination and Degradation in Basal-like Breast Cancer
    Authors: Y Kong, W Ren, H Fang, NA Shah, Y Shi, D You, C Zhou, D Jiang, C Yang, H Liang, W Liu, L Wang, W Gan, X Wan, F Li, Z Li, C Chen, N Xie
    International journal of biological sciences, 2022-02-28;18(5):2104-2115.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization
    Authors: S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas
    Cell Stem Cell, 2021-06-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells
    Authors: J Li, B Zhang, M Liu, X Fu, X Ci, J A, C Fu, G Dong, R Wu, Z Zhang, L Fu, JT Dong
    Cancers (Basel), 2020-03-21;12(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ChIP
  6. The aging skin microenvironment dictates stem cell behavior
    Authors: Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs
    Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020-02-24;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  8. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020-02-17;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  9. USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5
    Authors: Y Wu, J Qin, F Li, C Yang, Z Li, Z Zhou, H Zhang, Y Li, X Wang, R Liu, Q Tao, W Chen, C Chen
    J. Biol. Chem., 2019-10-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  10. HDAC-mediated deacetylation of KLF5 associates with its proteasomal degradation
    Authors: R Tao, B Zhang, Y Li, JL King, R Tian, S Xia, CR Schiavon, JT Dong
    Biochem. Biophys. Res. Commun., 2018-04-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, mmunoprecipitation
  11. Stem Cell Lineage Infidelity Drives Wound Repair and Cancer
    Authors: Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs
    Cell, 2017-04-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. KLF13 regulates the differentiation-dependent human papillomavirus life cycle in keratinocytes through STAT5 and IL-8
    Authors: W Zhang, S Hong, KP Maniar, S Cheng, C Jie, AW Rademaker, AM Krensky, C Clayberger
    Oncogene, 2016-04-04;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner.
    Authors: Shin J, Carr A, Corner G, Togel L, Davaos-Salas M, Tran H, Chueh A, Al-Obaidi S, Chionh F, Ahmed N, Buchanan D, Young J, Malo M, Hodin R, Arango D, Sieber O, Augenlicht L, Dhillon A, Weber T, Mariadason J
    J Biol Chem, 2014-07-18;289(36):25306-16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  14. Epithelial microRNAs regulate gut mucosal immunity via epithelium-T cell crosstalk.
    Authors: Biton M, Levin A, Slyper M, Alkalay I, Horwitz E, Mor H, Kredo-Russo S, Avnit-Sagi T, Cojocaru G, Zreik F, Bentwich Z, Poy MN, Artis D, Walker MD, Hornstein E, Pikarsky E, Ben-Neriah Y
    Nat. Immunol., 2011-01-30;12(3):239-46.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  15. KLF5 inhibition potentiates anti-PD1 efficacy by enhancing CD8+ T-cell-dependent antitumor immunity
    Authors: Q Wu, Z Liu, Z Gao, Y Luo, F Li, C Yang, T Wang, X Meng, H Chen, J Li, Y Kong, C Dong, S Sun, C Chen
    Theranostics, 2023-02-21;13(4):1381-1400.
  16. Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals
    Authors: Keisuke Fukuchi, Kayoko Nanai, Hiroshi Yuita, Chikako Maru, Jun Tsukada, Masato Ishigami et al.
    Journal of Oncology
  17. Kr�ppel-like factor 5 regulates stemness, lineage specification, and regeneration of intestinal epithelial stem cells
    Authors: CK Kim, M Saxena, K Maharjan, JJ Song, KR Shroyer, AB Bialkowska, RA Shivdasani, VW Yang
    Cell Mol Gastroenterol Hepatol, 2019-11-25;0(0):.

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