Human/Mouse Neurturin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Neurturin in Mouse Embryo. Neurturin was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse Neurturin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF477) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurturin
Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1 - 4). Similar to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active Neurturin is a disulfide-linked homodimer of the carboxy-terminal 100 amino acid residues. Mature mouse Neurturin shares 91% amino acid sequence similarity with human Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2, 5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1 - GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentration (5 - 8). Neurturin has been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems (9).
- Lin, L-FH. et al. (1993) Science 260:1130.
- Kotzbauer, P.T. et al. (1996) Nature 384:467.
- Milbrandt, J. et al. (1998) Neuron 20:245.
- Baloh, R.H. et al. (1998) Neuron 21:1291.
- Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
- Baloh, R.H. et al. (1997) Neuron 18:793.
- Jing, S. et al. (1996) Cell 85:1113.
- Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
- Airaksinen, M. and M. Saarma (2002) Nature Review Neuroscience 3:383.
Product Datasheets
Citations for Human/Mouse Neurturin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
Mol Ther Methods Clin Dev, 2018-02-23;9(0):172-180.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
Nature, 2014-07-27;514(7520):98-101.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Parasympathetic stimulation improves epithelial organ regeneration.
Authors: Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew
Nat Commun, 2013-01-01;4(0):1494.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC, Neutralization -
Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
Neuropharmacology, 2010-02-11;58(7):1114-21.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Developing therapeutically more efficient Neurturin variants for treatment of Parkinson's disease
Authors: Pia Runeberg-Roos, Elisa Piccinini, Anna-Maija Penttinen, Kert Mätlik, Hanna Heikkinen, Satu Kuure et al.
Neurobiology of Disease
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Role of neurturin in spontaneous itch and increased nonpeptidergic intraepidermal fiber density in a mouse model of psoriasis
Authors: Kent Sakai, Kristen M. Sanders, Marina R. Youssef, Kevin M. Yanushefski, Liselotte E. Jensen, Gil Yosipovitch et al.
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