Human/Mouse/Rat DARPP-32 Antibody Summary
Arg51-Ala204
Accession # Q9UD71
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat DARPP‑32 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DARPP-32 at approximately 32 - 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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DARPP‑32 in Human Brain. DARPP-32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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DARPP‑32 in Mouse Brain. DARPP-32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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DARPP‑32 in Rat Brain. DARPP-32 was detected in perfusion fixed frozen sections of rat brain (globus pallidus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DARPP-32
Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.
Product Datasheets
Citations for Human/Mouse/Rat DARPP-32 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
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Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice
Authors: Helseth AR, Hernandez-Martinez R, Hall VL et al.
Science (New York, N.Y.)
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Interleukin-9 protects from microglia- and TNF-mediated synaptotoxicity in experimental multiple sclerosis
Authors: Guadalupi, L;Vanni, V;Balletta, S;Caioli, S;De Vito, F;Fresegna, D;Sanna, K;Nencini, M;Donninelli, G;Volpe, E;Mariani, F;Battistini, L;Stampanoni Bassi, M;Gilio, L;Bruno, A;Dolcetti, E;Buttari, F;Mandolesi, G;Centonze, D;Musella, A;
Journal of neuroinflammation
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Dopaminergic Input Regulates the Sensitivity of Indirect Pathway Striatal Spiny Neurons to Brain-Derived Neurotrophic Factor
Authors: Ayon-Olivas, M;Wolf, D;Andreska, T;Granado, N;Lüningschrör, P;Ip, CW;Moratalla, R;Sendtner, M;
Biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Alpha‐Synuclein is Involved in DYT1 Dystonia Striatal Synaptic Dysfunction
Authors: Giulia Ponterio, Gaia Faustini, Ilham El Atiallah, Giuseppe Sciamanna, Maria Meringolo, Annalisa Tassone et al.
Movement Disorders
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CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington’s Disease Mouse Model
Authors: Freja K. Ekman, David S. Ojala, Maroof M. Adil, Paola A. Lopez, David V. Schaffer, Thomas Gaj
Molecular Therapy - Nucleic Acids
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Early structural and functional plasticity alterations in a susceptibility period of DYT1 dystonia mouse striatum
Authors: M Maltese, J Stanic, A Tassone, G Sciamanna, G Ponterio, V Vanni, G Martella, P Imbriani, P Bonsi, NB Mercuri, F Gardoni, A Pisani
Elife, 2018-03-05;7(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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