Human/Mouse/Rat eEF-2 Antibody

Catalog #: AF3806 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3806-SP	Ships in 5-7 Days	25 ug / $149
AF3806	In Stock	100 ug / $469

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Detection of Human/Mouse/Rat eEF‑2 by Western Blot.

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All eEF-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat eEF-2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat eEF-2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human eEF-2
Val300-Ser500
Accession # Q6PK56

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat eEF‑2 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat eEF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eEF-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eEF-2

The eukaryotic translation elongation factor-2 (eEF-2) is a GTP-binding protein that is required for the translocation step from the A site to the P site of the ribosome. Phosphorylation of eEF-2 by the eEF-2 kinase causes complete inhibition of its activity.

Long Name

Eukaryotic Translation Elongation Factor 2

Entrez Gene IDs

1938 (Human); 13629 (Mouse); 29565 (Rat)

Alternate Names

eEF2; eEF-2; EF-2; EF2EEF-2; elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase