Human/Mouse/Rat ERK2 Antibody

Catalog #: AF12301 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF12301
AF12301-SP

Detection of Human and Mouse ERK2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ERK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ERK2. Also recognizes ERK1 in some cell types at much lower affinity.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ERK2
Met1-Ser360
Accession # P28482

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

3-15 µg/mL

See below

Knockout Validated

ERK2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in ERK2 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse ERK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, TS1 mouse helper T cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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ERK2 in Human Breast. ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12301) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Knockout Validated

Western Blot Shows Human ERK2 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human ERK2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name

Extracellular Signal-regulated Kinase 2

Entrez Gene IDs

5594 (Human); 26413 (Mouse); 116590 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.24; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2