Home / p70 S6 Kinase / Human/Mouse/Rat p70 S6 Kinase Antibody

Human/Mouse/Rat p70 S6 Kinase Antibody

Catalog #: MAB8962
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8962
MAB8962-SP
Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat p70 S6 Kinase Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p70 S6 Kinase and p85 S6 Kinase. Reactivity with beta isoforms of p70 S6 Kinase is unknown.
Source
Monoclonal Mouse IgG1 Clone # 215247
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p70 S6 Kinase
Accession # M60725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of p70 S6 Kinase antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat p70 S6 Kinase Monoclonal Antibody (Catalog # MAB8962, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Western Blot Detection of Human/Mouse/Rat p70 S6 Kinase antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat p70 S6 Kinase by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.25 µg/mL Human/Mouse/Rat p70 S6 Kinase Monoclonal Antibody (Catalog # MAB8962) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active p70 S6 Kinase (Catalog # 896-KS) was included. A specific band for p70 S6 Kinase was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20‑fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well asT389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1).

References
  1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
  2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
Entrez Gene IDs
6198 (Human); 72508 (Mouse); 83840 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A

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Citations for Human/Mouse/Rat p70 S6 Kinase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
    Authors: T Walle, JA Kraske, B Liao, B Lenoir, C Timke, E von Bohlen, F Tran, P Griebel, D Albrecht, A Ahmed, M Suarez-Car, A Jiménez-Sá, T Beikert, A Tietz-Dahl, AN Menevse, G Schmidt, M Brom, JHW Pahl, W Antonopoul, M Miller, RL Perez, F Bestvater, NA Giese, P Beckhove, P Rosenstiel, D Jäger, O Strobel, D Pe'er, N Halama, J Debus, A Cerwenka, PE Huber
    Science Advances, 2022-03-23;8(12):eabh4050.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence
    Authors: E Lieschke, Z Wang, C Chang, CE Weeden, GL Kelly, A Strasser
    Cell Death and Differentiation, 2022-03-09;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. WYE-354 restores Adriamycin sensitivity in multidrug-resistant acute myeloid leukemia cell lines
    Authors: Sara M. Ibrahim, Sherin Bakhashab, Asad M. Ilyas, Peter N. Pushparaj, Sajjad Karim, Jalaluddin A. Khan et al.
    Oncology Reports

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Human/Mouse/Rat p70 S6 Kinase Antibody
By Jun Zhang on 03/16/2019
Application: WB Sample Tested: MAC-T mammary gland epithelial cell line Species: Bovine
Western Blot Human/Mouse/Rat p70 S6 Kinase Antibody MAB8962