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Human/Mouse/Rat PKC alpha Antibody

Catalog #: AF5340
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5340
AF5340-SP
Detection of Human/Mouse/Rat PKC alpha by Western Blot.
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Product Details
Citations (4)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKC alpha Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat PKC alpha in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PKC alpha
Lys604-Val672
Accession # P17252
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
Saos-2 human osteosarcoma cell line
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PKCa antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PKC alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Saos-2 human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKCa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKCa at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PKCa antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PKC alpha in Human Breast Cancer Tissue. PKCa was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human/Mouse/Rat PKCa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human PKC alpha by Simple WesternTM. Simple Western shows lysates of Saos‑2 human osteosarcoma cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC alpha at approximately 84 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PKC alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC alpha

PKC alpha (Protein kinase C alpha) is an 80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is widely expressed, and serves multiple cell-specific functions. PKC alpha is activated by increased diacylglycerol and intracellular calcium, and translocates to several sites such as the Golgi, nucleus and plasma membrane. Human PKC alpha is 672 amino acids (aa) in length. It contains two zinc finger regions (aa 36‑151), a protein kinase domain (aa 339‑557), and an AGC kinase region (aa 598‑668). Phosphorylations on Thr497, Thr638 and Ser657 are necessary for kinase activity. Over aa 604‑672, human PKC alpha shows 100% aa identity to mouse PKC alpha.

Long Name
Protein Kinase C alpha
Entrez Gene IDs
5578 (Human); 18750 (Mouse); 24680 (Rat); 447031 (Xenopus)
Alternate Names
AAG6; aging-associated gene 6; EC 2.7.11; EC 2.7.11.13; MGC129901; PKC alpha; PKCA; PKC-A; PKC-alpha; PKCAMGC129900; PRKACA; PRKCA; protein kinase C alpha type; protein kinase C, alpha

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Citations for Human/Mouse/Rat PKC alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Rod Bipolar Cells Require Horizontal Cells for Invagination Into the Terminals of Rod Photoreceptors
    Authors: Lena Nemitz, Karin Dedek, Ulrike Janssen-Bienhold
    Frontiers in Cellular Neuroscience
  2. Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy
    Authors: Suva Roy, Depeng Wang, Andra M. Rudzite, Benjamin Perry, Miranda L. Scalabrino, Mishek Thapa et al.
    Cell Reports Methods
  3. Electroacupuncture Inhibits the Activity of Astrocytes in Spinal Cord in Rats with Visceral Hypersensitivity by Inhibiting P2Y1 Receptor-Mediated MAPK/ERK Signaling Pathway
    Authors: J Zhao, H Li, C Shi, T Yang, B Xu
    Evid Based Complement Alternat Med, 2020-02-25;2020(0):4956179.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Spinal PKC? inhibition and gene-silencing for pain relief: AMPAR trafficking at the synapses between primary afferents and sensory interneurons
    Authors: O Kopach, V Krotov, A Shysh, A Sotnic, V Viatchenko, V Dosenko, N Voitenko
    Sci Rep, 2018-07-06;8(1):10285.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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