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Human/Mouse/Rat XPD Antibody

Catalog #: AF3296
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3296
AF3296-SP
Detection of Human/Mouse/Rat XPD by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat XPD Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat XPD in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human XPD
Met1-Gly360
Accession # P18074
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat XPD antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat XPD by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XPD

The Xeroderma Pigmentosum D (XPD) protein is a DNA helicase that acts as a subunit of the RNA polymerase II complex during nucleotide excision repair (NER) and transcription-coupled repair (TCR). XPD is also involved in p53-mediated apoptosis. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.

Long Name
Xeroderma Pigmentosum D
Entrez Gene IDs
2068 (Human); 13871 (Mouse); 308415 (Rat)
Alternate Names
Basic transcription factor 2 80 kDa subunit; BTF2 p80; COFS2; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; EC 3.6.1; EC 3.6.4.12; EM9; EM9TFIIH basal transcription factor complex 80 kDa subunit; ERCC2; excision repair cross-complementing rodent repair deficiency, complementationgroup 2; MAG; MGC102762; MGC126218; MGC126219; TFIIH 80 kDa subunit; TFIIH basal transcription factor complex helicase subunit; TFIIH basal transcription factor complex helicase XPD subunit; TTD; xeroderma pigmentosum complementary group D; Xeroderma pigmentosum group D-complementing protein; XPD; XPDC; XPDTFIIH p80

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Citation for Human/Mouse/Rat XPD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MAD2 interacts with DNA repair proteins and negatively regulates DNA damage repair.
    Authors: Fung MK, Han HY, Leung SC, Cheung HW, Cheung AL, Wong YC, Ling MT, Wang X
    J. Mol. Biol., 2008-06-05;381(1):24-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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