Human/Mouse Wnt-5a Antibody Summary
Gln38-Lys380
Accession # P22725
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Detection of Mouse Wnt-5a by Immunocytochemistry/Immunofluorescence WNT-5A expression in mouse GFAP+astrocytes. (A) Immunohistochemistry was performed on adult mouse brain sections using an anti-WNT-5A antibody in combination with anti-glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor protein1 (IBA1) antibodies as astrocyte and microglia marker, respectively. Merge presents the overlay of IBA1, GFAP, WNT-5A. Size bar −2 μm. The images represent a maximum intensity projection of a Z-stack of 5 μm thickness. The white square marked ‘B’ indicates the area magnified in B: (B) Close up of a GFAP+ astrocyte reveals the expression of WNT-5A in this cell type. (C) shows immunoblot detection of recombinant WNT-5A (rWNT-5A; 375 ng/lane) in comparison to lysates from mouse primary microglia and mixed astrocyte cultures. beta -actin serves as a loading control. (D) The bar graph depicts expression levels of WNT-5A mRNA in mouse primary microglia and mixed astrocyte cultures measured by QPCR. Data are normalized to GAPDH expression and analyzed with a non-parametric Mann–Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 4 to 8. (E) shows indirect immunocytochemistry of mixed astrocyte cultures employing anti-GFAP as astrocyte and anti-CD11b as microglia markers. DAPI is used as nuclear counterstain. Image represents a maximum image projection of an 8 μm Z-stack. Size bar 20 μm. The frame shows 63 cells in total and 10 CD11b-positive microglia (arrows). Routinely 10% to 18% microglia were observed (n = 4). DAPI, 4',6-diamidino-2-phenylindole; GADPH, glyceraldehyde 3-phosphate dehydrogenase; n, number. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Wnt-5a by Western Blot Wnt5a expressing tumors have less Wnt/ beta -catenin signaling than MMTV-Wnt1 tumors.(A) Quantitative RT-PCR of Wnt/ beta -catenin target genes. Expression of Axin2 mRNA in MMTV-Wnt1 versus MMTV-Wnt1;MMTV-Wnt5a tumors as determined by quantitative RT-PCR (n = 5 MMTV-Wnt1, n = 5 MMTV-Wnt1;MMTV-Wnt5a). Data are shown as tables obtained using REST software. Axin2 mRNA was significantly down-regulated in MMTV-Wnt1;MMTV-Wnt5a tumors. (B) Western blot for beta -catenin protein. Protein lysates were prepared from MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a tumors. beta -catenin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as loading controls. The ratio of active beta -catenin to beta -catenin as determined by densitometic analysis is shown. MMTV-Wnt1;MMTV-Wnt5a tumors displayed decreased levels of active beta -catenin compared to controls. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Wnt-5a by Western Blot Ectopic expression of Wnt5a results in low expression of K6 and K14.Expression of molecular markers for basal and luminal progenitors in MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mammary glands was compared by western blot. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Each lane contains protein isolated from a separate mouse. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Wnt-5a by Immunocytochemistry/Immunofluorescence WNT-5A expression in mouse GFAP+astrocytes. (A) Immunohistochemistry was performed on adult mouse brain sections using an anti-WNT-5A antibody in combination with anti-glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor protein1 (IBA1) antibodies as astrocyte and microglia marker, respectively. Merge presents the overlay of IBA1, GFAP, WNT-5A. Size bar −2 μm. The images represent a maximum intensity projection of a Z-stack of 5 μm thickness. The white square marked ‘B’ indicates the area magnified in B: (B) Close up of a GFAP+ astrocyte reveals the expression of WNT-5A in this cell type. (C) shows immunoblot detection of recombinant WNT-5A (rWNT-5A; 375 ng/lane) in comparison to lysates from mouse primary microglia and mixed astrocyte cultures. beta -actin serves as a loading control. (D) The bar graph depicts expression levels of WNT-5A mRNA in mouse primary microglia and mixed astrocyte cultures measured by QPCR. Data are normalized to GAPDH expression and analyzed with a non-parametric Mann–Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 4 to 8. (E) shows indirect immunocytochemistry of mixed astrocyte cultures employing anti-GFAP as astrocyte and anti-CD11b as microglia markers. DAPI is used as nuclear counterstain. Image represents a maximum image projection of an 8 μm Z-stack. Size bar 20 μm. The frame shows 63 cells in total and 10 CD11b-positive microglia (arrows). Routinely 10% to 18% microglia were observed (n = 4). DAPI, 4',6-diamidino-2-phenylindole; GADPH, glyceraldehyde 3-phosphate dehydrogenase; n, number. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Wnt-5a by Western Blot WNT-5A expression in mouse GFAP+astrocytes. (A) Immunohistochemistry was performed on adult mouse brain sections using an anti-WNT-5A antibody in combination with anti-glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor protein1 (IBA1) antibodies as astrocyte and microglia marker, respectively. Merge presents the overlay of IBA1, GFAP, WNT-5A. Size bar −2 μm. The images represent a maximum intensity projection of a Z-stack of 5 μm thickness. The white square marked ‘B’ indicates the area magnified in B: (B) Close up of a GFAP+ astrocyte reveals the expression of WNT-5A in this cell type. (C) shows immunoblot detection of recombinant WNT-5A (rWNT-5A; 375 ng/lane) in comparison to lysates from mouse primary microglia and mixed astrocyte cultures. beta -actin serves as a loading control. (D) The bar graph depicts expression levels of WNT-5A mRNA in mouse primary microglia and mixed astrocyte cultures measured by QPCR. Data are normalized to GAPDH expression and analyzed with a non-parametric Mann–Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 4 to 8. (E) shows indirect immunocytochemistry of mixed astrocyte cultures employing anti-GFAP as astrocyte and anti-CD11b as microglia markers. DAPI is used as nuclear counterstain. Image represents a maximum image projection of an 8 μm Z-stack. Size bar 20 μm. The frame shows 63 cells in total and 10 CD11b-positive microglia (arrows). Routinely 10% to 18% microglia were observed (n = 4). DAPI, 4',6-diamidino-2-phenylindole; GADPH, glyceraldehyde 3-phosphate dehydrogenase; n, number. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Wnt-5a by Western Blot Wnt/ beta -catenin signaling is not down-regulated in Wnt5a expressing MMTV-Wnt1 glands.(A) Western blot analysis of beta -catenin protein in MMTV-Wnt1mammary gland. The level of active beta -catenin was compared in protein lysates from MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mammary glands. Total beta -catenin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as loading controls. The ratio of active beta -catenin-to-beta -catenin is shown at the bottom on the gel. Each lane represents a sample from a different mouse. (B) Quantitative RT-PCR of Axin2, a Wnt/ beta -catenin target gene, in unsorted primary mammary epithelial cells. Expression of Axin2 and hWnt5a mRNA in unsorted PMECs from MMTV-Wnt1;MMTV-Wnt5a vs. MMTV-Wnt1 mammary glands was determined by quantitative RT-PCR (n = 12 MMTV-Wnt1, n = 11 MMTV-Wnt1;MMTV-Wnt5a separate mice). Data are shown as tables obtained using REST analysis software. Expression = fold difference in MMTV-Wnt1;Wnt5a relative to MMTV-Wnt1 controls after normalization to Gapdh. (C) Quantitative RT-PCR of Axin2 in E-cadherin negative primary mammary epithelial cells. Expression of Axin2 and hWnt5a mRNA in E-cadherin (Ecad) negative PMECs from MMTV-Wnt1;MMTV-Wnt5a vs. MMTV-Wnt1 mammary glands was determined by quantitative RT-PCR (n = 2 MMTV-Wnt1, n = 2 MMTV-Wnt1;MMTV-Wnt5a separate mice). Data are shown as tables obtained using REST analysis software. Expression = fold difference in MMTV-Wnt1;Wnt5a relative to MMTV-Wnt1 controls after normalization to Gapdh. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Wnt-5a by Western Blot Wnt5a expressing tumors demonstrate a decrease in markers of the basal tumor subtype.(A) Western blot using protein lysates isolated from the epithelium of MMTV-Wnt1 and MMTV-1;MMTV-Wnt5a mammary tumors. The expression of molecular markers of basal and luminal tumor subtypes were compared. Keratin 6 and Keratin 5 were strongly down-regulated in Wnt5a expressing tumors. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (B–D) Immunostaining for K6. Sections from MMTV-Wnt1 (B) and MMTV-1;MMTV-Wnt5a (C) tumors were stained with anti-Keratin 6 antibody using immunofluorescence (K6 = green; nuclei = blue). The percentage of cells expressing K6 was determined and graphed (D). Values are means +/− standard error (n = 6 MMTV-Wnt1, 3 fields per tumor; n = 5 MMTV-Wnt1;MMTV-Wnt5a, 3 fields per tumor). MMTV-Wnt1;MMTV-Wnt5a tumors demonstrated a significant decrease in K6-expressing cells as measured by T-test (* = p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-5a
Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23‑24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1‑2).
Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.
- Miller, J.R. (2002) Genome Biol. 3:3001.
- Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
- Veeman, M.T. et al. (2003) Developmental Cell 5:367.
- Topol, L. et al. (2003) J. Cell Biol 162:899.
- Torres, M. et al. (1996) J. Cell Biol. 133:1123.
- Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Product Datasheets
Citations for Human/Mouse Wnt-5a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cirmtuzumab blocks Wnt5a/ROR1 stimulation of NF-kappa B to repress autocrine STAT3 activation in chronic lymphocytic leukemia
Authors: Yun Chen, Liguang Chen, Jian Yu, Emanuela M. Ghia, Michael Y. Choi, Ling Zhang et al.
Blood
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Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants
Authors: João M. A. Santos, Leonardo Mendes-Silva, Vanessa Afonso, Gil Martins, Rui S. R. Machado, João A. Lopes et al.
Cell Death & Disease
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A cellular and spatial map of the choroid plexus across brain ventricles and ages
Authors: Neil Dani, Rebecca H. Herbst, Cristin McCabe, Gilad S. Green, Karol Kaiser, Joshua P. Head et al.
Cell
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beta -catenin-independent WNT signaling and Ki67 in contrast to the estrogen receptor status are prognostic and associated with poor prognosis in breast cancer liver metastases
Authors: Annalen Bleckmann, Lena-Christin Conradi, Kerstin Menck, Nadine Annette Schmick, Antonia Schubert, Eva Rietkötter et al.
Clinical & Experimental Metastasis
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Spatial regulation of cell cohesion by Wnt5a during second heart field progenitor deployment
Authors: Ding Li, Tanvi Sinha, Rieko Ajima, Hwa-Seon Seo, Terry P. Yamaguchi, Jianbo Wang
Developmental Biology
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Deletion of Wnt5a in osteoclasts results in bone loss through decreased bone formation
Authors: Roberts JL, Liu G, Paglia DN et al.
Annals of the New York Academy of Sciences
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A fibronectin gradient remodels mixed-phase mesoderm
Authors: Zhu, M;Gu, B;Thomas, EC;Huang, Y;Kim, YK;Tao, H;Yung, TM;Chen, X;Zhang, K;Woolaver, EK;Nevin, MR;Huang, X;Winklbauer, R;Rossant, J;Sun, Y;Hopyan, S;
Science advances
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium
Authors: Shu, Z;Fan, M;Tu, B;Tang, Z;Wang, H;Li, H;Li, H;Yuan, M;Bai, J;Huo, S;Wang, L;Zhu, WG;Wang, W;Liu, X;Shu, S;Zhao, Y;
Nature communications
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hyperglycemia increases SCO-spondin and Wnt5a secretion into the cerebrospinal fluid to regulate ependymal cell beating and glucose sensing
Authors: Nualart, F;Cifuentes, M;Ramírez, E;Martínez, F;Barahona, MJ;Ferrada, L;Saldivia, N;Bongarzone, ER;Thorens, B;Salazar, K;
PLoS biology
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Development of opioid-induced hyperalgesia depends on reactive astrocytes controlled by Wnt5a signaling
Authors: Liu X, Bae C, Liu B et al.
Molecular Psychiatry
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Increased MARCKS Activity in BRAF Inhibitor-Resistant Melanoma Cells Is Essential for Their Enhanced Metastatic Behavior Independent of Elevated WNT5A and IL-6 Signaling
Authors: V Yadav, N Jobe, SR Satapathy, P Mohapatra, T Andersson
Cancers, 2022-12-10;14(24):.
Species: Human
Sample Types: Protein Extracts
Applications: Western Blot -
YAP-dependent Wnt5a induction in hypertrophic adipocytes restrains adiposity
Authors: GJ Lee, YJ Kim, B Park, S Yim, C Park, H Roh, Y Moon, JK Seong, H Park
Cell Death & Disease, 2022-04-27;13(4):407.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
Authors: T Radaszkiew, M Nosková, K Gömöryová, O Vondálová, KA Radaszkiew, M Picková, R Víchová, T Gybe?, K Kaiser, L Demková, L Ku?erová, T Bárta, D Pot?šil, Z Zdráhal, K Sou?ek, V Bryja
Elife, 2021-10-27;10(0):.
Species: Human, Xenograft
Sample Types: Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Applications: Western Blot -
HDAC6 suppresses microRNA-199a transcription and augments HPV-positive cervical cancer progression through Wnt5a upregulation
Authors: Y Shao, F Zhu, S Zhu, L Bai
The international journal of biochemistry & cell biology, 2021-04-29;0(0):106000.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Myeloid-Derived Suppressor Cells Are a Major Source of Wnt5A in the Melanoma Microenvironment and Depend on Wnt5A for Full Suppressive Activity
Authors: Douglass SM, Fane ME, Sanseviero E et al.
Cancer Research
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Deletion of Wnt5a in osteoclasts results in bone loss through decreased bone formation
Authors: Roberts JL, Liu G, Paglia DN et al.
Annals of the New York Academy of Sciences
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Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
Authors: M Asem, AM Young, C Oyama, AG Claure De, Y Liu, J Yang, TS Hilliard, J Johnson, EI Harper, I Guldner, S Zhang, TM Page-Maybe, WJ Kaliney, MS Stack
Cancer Res., 2020-01-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells.
Authors: Hasan M, Rassenti L, Widhopf G, Yu J, Kipps T
Leukemia, 2018-12-19;33(3):653-661.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells
Authors: TC Huang, PT Lee, MH Wu, CC Huang, CY Ko, YC Lee, DY Lin, YW Cheng, KH Lee
PLoS ONE, 2017-08-31;12(8):e0181034.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
Respir. Res, 2017-03-24;18(1):52.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
Authors: Hoeke A Baarsma
J. Exp. Med, 2016-12-15;0(0):.
Species: Human, Mouse
Sample Types: In Vivo, Tissue Homogenates
Applications: Neutralization, Western Blot -
WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
J Exp Clin Cancer Res, 2016-09-13;35(1):144.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
Authors: Fuster J, Zuriaga M, Ngo D, Farb M, Aprahamian T, Yamaguchi T, Gokce N, Walsh K
Diabetes, 2014-10-28;64(4):1235-48.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
Sci Rep, 2014-09-10;4(0):6315.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
Authors: Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, Zhou X, Ye L
J Biol Chem, 2014-07-25;289(30):21028-39.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression.
Authors: Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T
Mol Oncol, 2014-05-27;8(8):1365-78.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
Authors: Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
J Invest Dermatol, 2014-03-21;134(8):2183-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma.
Authors: Yap L, Ahmad M, Zabidi M, Chu T, Chai S, Lee H, Lim P, Wei W, Dawson C, Teo S, Khoo A
Int J Oncol, 2014-03-13;44(5):1774-80.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
Authors: Barbolina MV, Burkhalter RJ, Stack MS
Biochem. J., 2011-07-01;437(1):1-12.
Species: Human
Sample Types: Ascites Fluid
Applications: Western Blot -
Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis
Authors: Shreyasi Maity, Arijit Chakraborty, Sushil Kumar Mahata, Syamal Roy, Anjan Kumar Das, Malini Sen
Frontiers in Immunology
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Aberrantly expressed Wnt5a in nurse-like cells drives resistance to Venetoclax in chronic lymphocytic leukemia
Authors: Yao Guo, Hanzhong Pei, Bo Lu, Dengyang Zhang, Yuming Zhao, Fuqun Wu et al.
Cell Death Discovery
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Development of opioid-induced hyperalgesia depends on reactive astrocytes controlled by Wnt5a signaling
Authors: Liu X, Bae C, Liu B et al.
Molecular Psychiatry
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Myeloid-Derived Suppressor Cells Are a Major Source of Wnt5A in the Melanoma Microenvironment and Depend on Wnt5A for Full Suppressive Activity
Authors: Douglass SM, Fane ME, Sanseviero E et al.
Cancer Research
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Wnt5a induces ROR1/ROR2 heterooligomerization to enhance leukemia chemotaxis and proliferation
Authors: Jian Yu, Liguang Chen, Bing Cui, George F. Widhopf, Zhouxin Shen, Rongrong Wu et al.
Journal of Clinical Investigation
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FOXC1-induced non-canonical WNT5A-MMP7 signaling regulates invasiveness in triple-negative breast cancer
Authors: B Han, B Zhou, Y Qu, B Gao, Y Xu, S Chung, H Tanaka, W Yang, AE Giuliano, X Cui
Oncogene, 2017-12-18;0(0):.
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Increased Extracellular Vesicles Mediate WNT5A Signaling in Idiopathic Pulmonary Fibrosis
Authors: Aina Martin-Medina, Mareike Lehmann, Olivier Burgy, Sarah Hermann, Hoeke A. Baarsma, Darcy E. Wagner et al.
American Journal of Respiratory and Critical Care Medicine
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Characterisation of tumour-derived microvesicles in cancer patients’ blood and correlation with clinical outcome
Authors: Kerstin Menck, Annalen Bleckmann, Astrid Wachter, Bianca Hennies, Lena Ries, Matthias Schulz et al.
Journal of Extracellular Vesicles
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Heterotrimeric G protein-dependent WNT-5A signaling to ERK1/2 mediates distinct aspects of microglia proinflammatory transformation
Authors: Carina Halleskog, Jacomijn Petronella Dijksterhuis, Michaela Brita Christina Kilander, Javier Becerril-Ortega, Juan Carlos Villaescusa, Eva Lindgren et al.
Journal of Neuroinflammation
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Induction and transport of Wnt 5a during macrophage-induced malignant invasion is mediated by two types of extracellular vesicles
Authors: Kerstin Menck, Florian Klemm, Julia Christina Gross, Tobias Pukrop, Dirk Wenzel, Claudia Binder
Oncotarget
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Wnt5a and Wnt11 as acute respiratory distress syndrome biomarkers for severe acute respiratory syndrome coronavirus 2 patients
Authors: Eun Young Choi, Hee Ho Park, Hyelim Kim, Hong Nam Kim, Inyoung Kim, Soyoung Jeon et al.
European Respiratory Journal
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Wnt5a Suppresses Tumor Formation and Redirects Tumor Phenotype in MMTV-Wnt1 Tumors
Authors: Stephanie L. Easter, Elizabeth H. Mitchell, Sarah E. Baxley, Renee Desmond, Andra R. Frost, Rosa Serra
PLoS ONE
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Expression profile and clinical significance of Wnt signaling in human gliomas
Authors: Hao Zhang, Yanhua Qi, Decheng Geng, Yi Shi, Xu Wang, Rutong Yu et al.
Oncology Letters
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Noncanonical WNT Activation in Human Right Ventricular Heart Failure
Authors: Jonathan J. Edwards, Jeffrey Brandimarto, Dong-Qing Hu, Sunhye Jeong, Nora Yucel, Li Li et al.
Frontiers in Cardiovascular Medicine
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Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans
Authors: Oldoni F, van Capelleveen JC, Dalila N.
Arteriosclerosis, Thrombosis, and Vascular Biology
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Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed).
Concentration used - 8ug/mL.
