Human MyD88 Antibody

Catalog #: MAB29281 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29281
MAB29281-SP

Detection of MyD88 in Raji Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MyD88 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MyD88 in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse MyD88 is observed.

Source

Monoclonal Rat IgG_2B Clone # 316628

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MyD88
Met1-Pro296
Accession # Q99836

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MyD88 antibody in Raji Human Cell Line antibody by Flow Cytometry.

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Detection of MyD88 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB29281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyD88

Myeloid Differentiation primary response gene 88 (MyD88) contains a cytoplasmic Toll/interleukin 1 receptor (TIR) and functions as an adaptor protein in Toll-like receptor signaling. It is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.

Long Name

Myeloid Differentiation Primary Response Gene 88

Entrez Gene IDs

4615 (Human); 17874 (Mouse)

Alternate Names

MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88