Human NCK1 Antibody

Catalog #: AF7008 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7008
AF7008-SP

Detection of Human NCK1 by Western Blot.

1 Image

All NCK1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NCK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NCK1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NCK1
Ala2-Ser377
Accession # P16333

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human NCK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7008) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NCK1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NCK1

NCK1 (Non-catalytic region of tyrosine kinase 1; also Sh2/SH3 adaptor protein NCK-alpha ) is a 46-48 kDa member of the Nck family of adaptor proteins. NCK1 is ubiquitously expressed, and may be found in both cytoplasm and nucleus. In the cytoplasm, it connects tyrosine kinases to actin reorganization which, in T cells, involves SLP-76 and VAV1. Upon DNA damage, NCK1 binds SOCS7 and is translocated into the nucleus where it participates in p53 phosphorylation and cell cycle arrest. Human NCK1 is 377 amino acids (aa) in length. It contains three N-terminal SH3 domains (aa 2-252) that mediate binding to Pro-rich regions of cytoplasmic proteins, and a C-terminal SH2 domain (aa 282-376) that binds to phosphorylated proteins or receptors. There are at least three utilized Ser, and one utilized Tyr phosphorylation sites. There are also three potential splice variants. One shows a 12 aa substitution for aa 1-76, a second possesses an 11 aa substitution for aa 77‑377, and a third contains a 37 aa substitution for aa 134-377. Full-length NCK1 shares 99% aa identity with mouse NCK1.

Long Name

Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1

Entrez Gene IDs

4690 (Human); 179731 (Mouse); 300955 (Rat)

Alternate Names

cytoplasmic protein NCK1; MGC12668; NCK adaptor protein 1melanoma NCK protein; NCK1; nck-1; NCKalpha; NCKNCK tyrosine kinase; non-catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK-alpha