Human NELL1 Antibody

Catalog #: MAB54871 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB54871
MAB54871-SP

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Human NELL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NELL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NELL2 or recombinant mouse NELL1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 756435

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NELL1
Arg17-Asn810
Accession # Q92832

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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NELL1 in IMR32 Human Cell Line. NELL1 was detected in immersion fixed IMR32 human neuroblastoma cell line using Mouse Anti-Human NELL1 Monoclonal Antibody (Catalog # MAB54871) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NELL1

NELL1 (neural EGF-like like protein 1) is an 89 kDa (predicted) member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. When secreted, NELL1 exists as a phosphoglycoprotein that can add as much as 50 kDa to the calculated MW. NELL1 has restricted expression, being limited to pre-B cells and osteoblasts, where it apparently promotes osteoblast maturation and bone formation. In tumors, it is found in neuroblastoma-derived cells. NELL1 is both secreted and retained intracellularly where it is phosphorylated by PKC. The human NELL1 precursor is 810 amino acids (aa) in length. It contains a 16 aa signal sequence plus a 794 aa mature region. The mature region possesses an N-terminal TSP domain (aa 81-230), two VWFC domains (aa 271-390), six consecutive EGF-like domains (aa 391-631), and three additional C-terminal VWFC domains (aa 632-807). Secreted NELL1 forms a 400-420 kDa noncovalent homotrimer. Over aa 17-810, human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternate splicing generates an additional isoform of human NELL1 that lacks the fifth EGF-like domain.

Long Name

NEL-like 1

Entrez Gene IDs

4745 (Human); 338352 (Mouse)

Alternate Names

FLJ45906; IDH3GL; nel (chicken)-like 1; NELL1; NEL-like 1 (chicken); NEL-like protein 1; Nel-related protein 1; neural epidermal growth factor-like 1; NRP1; protein kinase C-binding protein NELL1