Human Pappalysin-2/PAPP-A2 Antibody Summary
Ser234-Cys1396
Accession # Q9BXP8
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Pappalysin-2/PAPP-A2 by Western Blot Effects of gene disruption on PAPP-A2 protein.Shown are Western blots of 9 representative (A) placentae and (B) embryos at embryonic day 12.5 from embryos homozygous for wild-type Pappa2 (+/+), heterozygous (+/−) or homozygous for the disruption (−/−). The nitrocellulose membrane was scanned for fluorescence at 700 and 800 nm simultaneously, with fluorescence at 700 nm corresponding to actin (at approximately 40 kDa) and fluorescence at 800 nm corresponding to PAPP-A2 (at approximately 250 kDa). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23457539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pappalysin-2/PAPP-A2
Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.
- Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
- Boldt, H.B. et al. (2001) Biochem. J. 358:359.
- Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
- Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
Product Datasheets
Citations for Human Pappalysin-2/PAPP-A2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Unique Transcriptional Programs Identify Subtypes of AKI
Authors: Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al.
Journal of the American Society of Nephrology
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IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
Reprod Biol Endocrinol, 2014-12-04;12(0):123.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
First-Trimester Levels of Pregnancy-Associated Plasma Protein A2 (PAPP-A2) in the Maternal Circulation Are Elevated in Pregnancies That Subsequently Develop Preeclampsia
Authors: Erin J. Crosley, Ursula Durland, Ken Seethram, Scott MacRae, Andrée Gruslin, Julian K. Christians
Reproductive Sciences
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Pregnancy Associated Plasma Protein A2 (PAPP-A2) Affects Bone Size and Shape and Contributes to Natural Variation in Postnatal Growth in Mice
Authors: Julian Kenneth Christians, Devin Rhys de Zwaan, Sunny Ho Yeung Fung
PLoS ONE
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Regulation of pregnancy-associated plasma protein A2 (PAPPA2) in a human placental trophoblast cell line (BeWo)
Authors: Pamela K Wagner, Aki Otomo, Julian K Christians
Reproductive Biology and Endocrinology
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Altered placental expression of PAPPA2 does not affect birth weight in mice
Authors: Pamela K Wagner, Julian K Christians
Reproductive Biology and Endocrinology
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
Authors: Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
J. Endocrinol., 2009-05-27;202(3):337-45.
Species: Human, Mouse
Sample Types: Serum, Whole Tissue
Applications: IHC-P, Western Blot -
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
Mol. Hum. Reprod., 2008-09-18;14(10):595-602.
Species: Human
Sample Types: Serum, Tissue Homogenates, Whole Tissue
Applications: ELISA Development, IHC-P, Western Blot
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