Human PDGFRL Antibody

Catalog #: MAB1723 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1723-100
MAB1723-SP

Detection of PDGFRL in A549 Human Cell Line by Flow Cytometry

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All PDGFRL Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGFRL Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGFRL in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1008804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human PDGFRL
Gln22-Ser375
Accession # Q15198

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

A549 human lung carcinoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of PDGFRL antibody in A549 Human Cell Line antibody by Flow Cytometry

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Detection of PDGFRL in A549 Human Cell Line by Flow Cytometry A549 human lung carcinoma cell line was stained with Mouse anti-Human PDGFRL Monoclonal Antibody (Catalog # MAB1723, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGFRL

Platelet-derived growth factor receptor-like protein is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, human PDGFRL is 90% similar to the mouse version and 91% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). PDGFRL is a tumor suppressor active in the tumor-suppression network and implicated in colorectal cancer, and a decrease in PDGFRL expression levels has been observed in breast cancer (1). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (6). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (7). Study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation.

References

Guo, F-J. et al. (2010) World J Gastroenterol. 16:1465.
Andrae, J. et al. (2008) Genes Dev. 22:1276.
Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
Matsui, T. et al. (1989) Science 243:800.
Hou, S. et al. (2013). Human Mutat. 34:74.
Fujikado, N. et al. (2006). Arthritis Res. & Ther. 8:R100.
Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Long Name

Platelet-derived Growth Factor Receptor-like

Entrez Gene IDs

5157 (Human); 68797 (Mouse); 290771 (Rat)

Alternate Names

PDGFRL; PDGFR-Like Protein; PDGRL; platelet-derived growth factor receptor-like protein; platelet-derived growth factor receptor-like; platelet-derived growth factor-beta-like tumor suppressor; PRLTS; PRLTSPDGF receptor beta-like tumor suppressor