Human PP2C gamma Antibody

Catalog #: AF5595 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5595
AF5595-SP

Detection of Human PP2C gamma by Western Blot.

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Human PP2C gamma Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human PP2C gamma in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PP2C gamma
Met1-Asp546
Accession # O15355

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PP2C gamma by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PP2C gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP2C gamma at approximately 72 to 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2C gamma

PP2C gamma (Protein phosphatase 2C gamma; also PPM1G) is a nuclear member of the metal ion-dependent PP2C family of Ser/Thr protein phosphatases. Although its predicted MW is 59 kDa, it runs anomalously at 72‑75 kDa in SDS-PAGE. PP2C gamma is widely expressed and serves as a negative regulator of the cell stress response. In particular, it dephosphorylates histones H2A and H2B, allowing for their placement into chromatin. This is important when histones are reincorporated into DNA following DNA repair. Human PP2C gamma is 546 amino acids (aa) in length. It contains a catalytic domain (aa 27‑120), an Asp/Glu-rich region (aa 258‑326) and a poly-Lys tail (aa 539‑544). One potential splice form shows a Phe substitution for aa 20‑38.

Long Name

Protein Phosphatase 2C gamma

Entrez Gene IDs

5496 (Human); 14208 (Mouse); 259229 (Rat)

Alternate Names

EC 3.1.3.16; MGC1675; MGC2870; PP2C gamma; PP2C, gamma; PP2CG; PP2CGAMMA; PP2C-gamma; PPM1C; PPM1G; PPP2CG; Protein phosphatase 1C; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 1G; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; Protein phosphatase 2C isoform gamma; Protein phosphatase magnesium-dependent 1 gamma; protein phosphatase, Mg2+/Mn2+ dependent, 1G