Human Prohibitin Antibody

Catalog #: AF3470 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3470
AF3470-SP

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Human Prohibitin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Prohibitin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Prohibitin
Ala2-Gln272
Accession # P35232

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Prohibitin

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Prohibitin in MCF‑7 Human Cell Line. Prohibitin was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human Prohibitin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prohibitin

Prohibitin is an approximately 30 kDa protein that belongs to the prohibitin family. It contains a characteristic prohibitin domain and has been shown to have multiple functions. It can be localized to the mitochondria, nucleus, plasma membrane, and is also present in circulation. Post translational modifications of Prohibitin, including phosphorylation and ubiquitination, also occurs. Human Prohibitin shares 99% amino acid sequence identity with the mouse or rat protein.

Entrez Gene IDs

5245 (Human); 18673 (Mouse); 25344 (Rat)

Alternate Names

PHB; PHB1; Prohibitin