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Human RANK/TNFRSF11A Antibody

Catalog #: AF683
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF683
AF683-SP
Human RANK/TNFRSF11A Antibody Agonist Activity
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RANK/TNFRSF11A Antibody Summary

Species Reactivity
Human
Specificity
Detects human RANK in direct ELISAs and Western blots. In these formats, approximately 30% cross‑reactivity with recombinant mouse RANK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RANK
Gln29-Gly213
Accession # Q9Y6Q6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)
Agonist Activity
Human RANK/TNFRSF11AAntibody (Catalog # AF683) induces osteoclast differentiation of RAW 264.7mouse monocyte/macrophage cells in the presence of recombinant mouse M-CSF(Catalog # 416ML). The ED50 for this effect is typically 0.0750 -1.50 μg/mL.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity View Larger

Human RANK/TNFRSF11A Antibody Agonist Activity Human RANK/TNFRSF11A Antibody (Catalog # AF683) induces osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells in the presence of recombinant mouse M-CSF (416ML). The ED50 for this effect is typically 0.0750 – 1.50 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References
  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
Long Name
Receptor Activator of NF-kappa-B
Entrez Gene IDs
8792 (Human); 21934 (Mouse)
Alternate Names
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

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Citations for Human RANK/TNFRSF11A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Analysis of RANK-c interaction partners identifies TRAF3 as a critical regulator of breast cancer aggressiveness
    Authors: Sirinian C, Papanastasiou AD, Karayel O et al.
    Neoplasia
  2. RANK signaling increases after anti-HER2 therapy contributing to the emergence of resistance in HER2-positive breast cancer
    Authors: Adrián Sanz-Moreno, Sonia Palomeras, Kim Pedersen, Beatriz Morancho, Tomas Pascual, Patricia Galván et al.
    Breast Cancer Research
  3. The Intrinsic and Extrinsic Implications of RANKL/RANK Signaling in Osteosarcoma: From Tumor Initiation to Lung Metastases
    Authors: Benjamin Navet, Kosei Ando, Jorge William Vargas-Franco, Régis Brion, Jérome Amiaud, Kanji Mori et al.
    Cancers (Basel)
  4. Identification of novel human receptor activator of nuclear factor-kB isoforms generated through alternative splicing: implications in breast cancer cell survival and migration
    Authors: Anastasios D Papanastasiou, Chaido Sirinian, Haralabos P Kalofonos
    Breast Cancer Research
  5. IL-3 inhibits human osteoclastogenesis and bone resorption through downregulation of c-Fms and diverts the cells to dendritic cell lineage.
    Authors: Gupta N, Barhanpurkar AP, Tomar GB
    J. Immunol., 2010-07-19;185(4):2261-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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