Human/Rat Fgr Antibody

Catalog #: AF3207 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3207
AF3207-SP

Detection of Human/Rat Fgr by Western Blot.

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Human/Rat Fgr Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat Fgr in Western blot.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

Immersion fixed human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Rat Fgr by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Fgr Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3207) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Fgr at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Fgr in U937 Human Cell Line. Fgr was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human/Rat Fgr Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3207) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fgr

Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of nonreceptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.

Long Name

Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog

Entrez Gene IDs

2268 (Human); 14191 (Mouse); 79113 (Rat)

Alternate Names

c-fgr; c-src-2 proto-oncogene; c-src2; EC 2.7.10; EC 2.7.10.2; Fgr; FLJ43153; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; MGC75096; p55-c-fgr protein; p55c-fgr; p55-Fgr; p58c-fgr; Proto-oncogene c-Fgr; proto-oncogene tyrosine-protein kinase FGR; Src2; SRC2c-fgr protooncogene; tyrosine-protein kinase Fgr