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Human/Rat SUSD2 Antibody

Catalog #: MAB90563 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB90563-SP
MAB90563-100
Detection of SUSD2 by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat SUSD2 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human SUSD2 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1279D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HEK293 human embryonic kidney cell line-derived human SUSD2
Met1-Ala785
Accession # Q9UGT4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of SUSD2 antibody by Western Blot. View Larger

Detection of SUSD2 by Western Blot. Western blot shows lysates of SK-BR-3 human breast cancer cell line, human lung tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Rat SUSD2 Monoclonal Antibody (Catalog # MAB90563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for SUSD2 at approximately 110 and 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of SUSD2 antibody in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP antibody by Flow Cytometry. View Larger

Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human/Rat SUSD2 Monoclonal Antibody (Catalog # MAB90563) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SUSD2

Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective Isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibits colon cancer cell growth through inducing G1 arrest. There is  evidence that SUSD2 may play a role in breast tumorigenesis.

Long Name
Sushi Domain Containing 2
Entrez Gene IDs
56241 (Human); 71733 (Mouse); 294335 (Rat)
Alternate Names
BK65A6.2; FLJ22778; SUSD2; Sushi domain (SCR repeat) containing; sushi domain containing 2; sushi domain-containing protein 2

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