Human Reg4 Antibody Summary
Asp23-Pro158
Accession # Q9BYZ8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg4
Reg4 (regenerating islet-derived protein 4; also named Reg-like protein, RELP, and gastrointestinal secretory protein) is a 17 kDa, secreted glycoprotein belonging to the calcium (C-type) dependent lectin superfamily and the regenerating gene family (1‑3). There are three potential isoforms for human Reg4. Isoform 1 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain. Amino acids 30‑156 constitute a C-type lectin-like domain, and aa 50 is a site for potential N-linked glycosylation. In addition, amino acids 127 and 142‑143 form a ligand-binding surface. In isoform 2, there is a 79 aa substitution for the C-terminal 101 amino acids, while in isoform 3, there is an 11 aa substitution for the C‑terminal 57 amino acids. Human Reg4 shares 68% and 67% aa sequence identity with rat and mouse Reg4, respectively. Like other members of the regenerating gene family, Reg4 is expressed in the gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury (1, 2). In particular, Reg4 is expressed in normal colon mucosa, and up‑regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and, outside the GI tract, in prostate adenocarcinoma (2‑8). The physiological function of Reg4 is presently unknown, but it may be involved in inflammatory and metaplastic responses of the GI epithelium (3).
- Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
- Zhang, Y. et al. (2003) World J. Gastroenterol. 9:2635.
- Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
- Zhang, Y. et al. (2003) Cancer Lett. 200:69.
- Violette, S. et al. (2003) Int. J. Cancer 103:185.
- Oue, N. et al. (2005) J. Pathol. 207:185.
- Zhennan, G. et al. (2005) Clin. Cancer Res. 2:2237
- Bishnupuri, K.S. et al. (2006) Gastroenterology 130:137.
Product Datasheets
Citations for Human Reg4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells.
Authors: Rafa L, Dessein AF, Devisme L, Buob D, Truant S, Porchet N, Huet G, Buisine MP, Lesuffleur T
Int. J. Oncol., 2010-03-01;36(3):689-98.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Reg IV is a serum biomarker for gastric cancer patients and predicts response to 5-fluorouracil-based chemotherapy.
Authors: Mitani Y, Oue N, Matsumura S, Yoshida K, Noguchi T, Ito M, Tanaka S, Kuniyasu H, Kamata N, Yasui W
Oncogene, 2007-01-22;26(30):4383-93.
Species: Human
Sample Types: Serum
Applications: ELISA Development
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
We used this antibody for a sandwich ELISA format in combination with pAb (AF1379) and protein (1379-RG). This combination worked very well and detected human Reg 4 in serum and plasma samples.
