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Human Ret Antibody

Catalog #: MAB718
4 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB718-SP
MAB718-500
MAB718-100
Detection of Ret in SH‑SY5Y Human Cell Line by Flow Cytometry.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ret Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ret in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Ret is observed.
Source
Monoclonal Mouse IgG1 Clone # 132507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Ret/Fc Chimera
Leu29-Arg635
Accession # P07949
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Ret (Catalog # 1168-CR)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Ret antibody in SH‑SY5Y Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Ret in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Ret Monoclonal Antibody (Catalog # MAB718, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1‑4). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1‑4).

References
  1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
  4. Baloh, R. et al. (1998) Neuron 21:1291.
Long Name
Proto-oncogene Tyrosine-protein Kinase Receptor Ret
Entrez Gene IDs
5979 (Human); 19713 (Mouse)
Alternate Names
CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret

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Citations for Human Ret Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
    Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
    Cell Stem Cell, 2023-03-02;30(3):264-282.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
    Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
    Cell Stem Cell, 2023;30(3):264-282.e9.
  3. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014-07-27;514(7520):98-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
    FASEB J., 2011-04-27;25(0):2604-14.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human Ret Antibody

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Human Ret Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human Ret Antibody MAB718
Human Ret Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human