Human ROR1 Antibody

Detection of ROR1 by Western Blot. Western blot shows lysate of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). View our protocol for Staining Membrane-associated Proteins.

Detection of Human ROR1 by Simple Western^TM. Simple Western lane view shows lysates of NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human ROR1 by Knockdown Validated Knockdown of ROR1 in serous ovarian cancer cells decreases migrationA. ROR1 is decreased at the mRNA level following ROR1 siRNA (A) induced knockdown in serous ovarian cancer (OVCAR3) cells. No effect on ROR2 mRNA level. qRT-PCR was performed in triplicate and normalised to three different housekeeping genes (SDHA, HSPCB, RPL13A). Results represent an average of three experiments. Error bars represent the s.d of the mean. **P < 0.01. B. Densitometric analysis of ROR1 and ROR2 protein levels from three separate experiments. Representative immunoblots showing ROR1 knockdown at the protein level in OVCAR3 cells. No effect on ROR2 protein level. Top panel: ROR1, middle panel: ROR2, bottom panel: alpha -tubulin. C. Cell proliferation does not change following ROR1 knockdown in OVCAR3 cells over a 48–72 hour period. Results represent the average of three independent experiments. Error bars represent the s.d of the mean. D. ROR1 knockdown has no effect on the adhesion of OVCAR3 cells to collagen or fibronectin. Results represent the average of 3 experiments and error bars represent the s.d of the mean. E. Cell migration performed using the wound healing assay decreases following ROR1 knockdown in OVCAR3 cells. Results represent an average of three experiments. Error bars represent the s.d of the mean. *P < 0.05. F. Relative cell migration performed using the transwell migration assay is significantly decreased following ROR1 knockdown in OVCAR3 cells. Results represent an average of three experiments. Error bars represent the s.d of the mean. **P < 0.01. G. Relative cell invasion performed using the matrigel pre coated transwell assay is significantly decreased following ROR1 knockdown in OVCAR3 cells. Results represent the average of three experiments. Error bars represent the s.d of the mean. **P < 0.01. H. Representative picture of OVCAR3 cells invading matrigel over 48 hours. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26515598), licensed under a CC-BY license. Not internally tested by R&D Systems.