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Human S100A11 Antibody

Catalog #: AF4874
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4874
AF4874-SP
Detection of Human S100A11 by Western Blot.
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Citations (1)
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Human S100A11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) S100A1, A2, A7, A8, A10, A13, A16, rhS100B, rhS100P, recombinant mouse (rm) S100A6, and rmS100A10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human S100A11
Ala2-Thr105
Accession # P31949
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human S100A11 antibody by Western Blot. View Larger

Detection of Human S100A11 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4874) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A11

S100A11 (also S100C and calgizzarin) is a 10‑12 kDa member of the S100 family, EF-hand superfamily of Ca‑binding proteins. It is produced by smooth muscle and keratinocytes. Intracellularly, it suppresses growth; extracellularly, it exists as both a monomer, homodimer, and heterodimer with S100B, binds to RAGE, induces EGF, and promotes cell growth. Human S100A11 is 105 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑49 and 55‑90) and one high-affinity Ca‑binding site (aa 68‑79), and binds annexin I with its C-terminal half. There may be one alternative splice form that apparently contains a series of mutations over aa 61‑79 and shows 90% overall aa identity to the standard form. Full-length human S100A11 shares 78% and 82% aa identity with mouse and porcine S100A11, respectively.

Long Name
S100 Calcium Binding Protein A11
Entrez Gene IDs
6282 (Human); 20195 (Mouse); 445415 (Rat)
Alternate Names
Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70; MLN70; protein S100-A11; Protein S100-C; S100 calcium binding protein A11; S100 calcium-binding protein A11 (calgizzarin); S100 calcium-binding protein A11; S100A11; S100C; S100CS100 calcium binding protein A11 (calgizzarin)

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Citation for Human S100A11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Therapeutic potential of targeting S100A11 in malignant pleural mesothelioma
    Authors: H Sato, M Sakaguchi, H Yamamoto, S Tomida, K Aoe, K Shien, T Yoshioka, K Namba, H Torigoe, J Soh, K Tsukuda, H Tao, K Okabe, S Miyoshi, HI Pass, S Toyooka
    Oncogenesis, 2018-01-24;7(1):11.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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