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Human S100A2 Antibody

Catalog #: MAB48701 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB48701
MAB48701-SP
S100A2 in Human Prostate.
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Human S100A2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) S100A1, A7, A8, A10, A11, A13, rhS100B, rhS100P, recombinant mouse S100A4, A6, A9, or A16 is observed.
Source
Monoclonal Mouse IgG1 Clone # 674033
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human S100A2
Met1-Pro97
Accession # P29034
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry S100A2 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

S100A2 in Human Prostate. S100A2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human S100A2 Monoclonal Antibody (Catalog # MAB48701) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial, smooth muscle and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A2

S100A2 (also S100L and CaN19) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by multiple cell types, including keratinocytes, chondrocytes, and bronchial epithelium. S100A2 regulates both Ca and Zn (in human) within cells, and increases p53 activity. It forms both noncovalent and covalent homodimers, and a homotetramer under certain conditions. Human S100A2 is 98 amino acids (aa) in length. It contains two EF-hand motifs (aa 13-48 and 51-86) plus a calcium (aa 64-75) and zinc (aa 17-22) binding site. There is one potential alternative splice form that shows a 40 aa insertion after Gly48. Full-length human S100A2 shares 89% and 85% aa identity with bovine and canine S100A2, respectively.

Long Name
S100 Calcium Binding Protein A2
Entrez Gene IDs
6273 (Human)
Alternate Names
CAN19; CAN19protein S100-A2; MGC111539; S100 calcium binding protein A2; S100 calcium-binding protein A2Protein S-100L; S100A2; S100L

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