Home / Siglec-8 / Human Siglec-8 Antibody

Human Siglec-8 Antibody

Catalog #: MAB7975
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7975
MAB7975-SP
Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
Source
Monoclonal Mouse IgG1 Clone # 837535
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec-8 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Monoclonal Antibody (Catalog # MAB7975) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-8

Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory
CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.

Long Name
Sialic Acid Binding Ig-like Lectin 8
Entrez Gene IDs
27181 (Human)
Alternate Names
CD329 antigen; CDw329; MGC59785; SAF2; SAF2SAF-2; sialic acid binding Ig-like lectin 8; Sialoadhesin family member 2; Siglec8; Siglec-8; SIGLEC8L; SIGLEC8Lsialic acid-binding Ig-like lectin 8

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Citations for Human Siglec-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19
    Authors: Lucie Rodriguez, Pirkka T. Pekkarinen, Tadepally Lakshmikanth, Ziyang Tan, Camila Rosat Consiglio, Christian Pou et al.
    Cell Reports Medicine
  2. Discovery, Function, and Therapeutic Targeting of Siglec-8
    Authors: BA Youngblood, J Leung, R Falahati, J Williams, J Schanin, EC Brock, B Singh, AT Chang, JA O'Sullivan, RP Schleimer, N Tomasevic, CR Bebbington, BS Bochner
    Cells, 2020-12-24;10(1):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development

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