Human Skp2 Antibody Summary
Leu130-Pro350
Accession # Q13309
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Skp2 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Skp2 at approximately 45 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Detection of Human Skp2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for Skp2 at approximately 49 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Skp2
Skp2 (S-phase kinase-associated protein 2) is a 45-49 kDa cyclin A- and CDK2-associated phosphoprotein within the FBXL (F-box/LRR) family of ubiquitin ligases, designated FBXL1. Skp2 contains an F-box domain (amino acids (aa) 94-140) and 8 leucine-rich repeats (LRR) between aa 180 and 403. Isoforms of 424 and 410 aa, designated a and b, respectively, diverge after aa 354. As a component of the SCF (SKP1-Cul1-F-box) complex, Skp2 phosphorylation is required for degradation of CDK inhibitors such as p27/Kip1 and progression from G1 to S phases of the cell cycle. Deregulation in cancer promotes cell cycle progression and cancer growth. Within the region used as an immunogen, human Skp2 shares 84% aa sequence identity with mouse and rat Skp2.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Skp2 Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Human Skp2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
