Human Smad8 Antibody

Name: Human Smad8 Antibody
Brand: R&D Systems
SKU: AF2309
Rating: 4 (2 reviews)

Catalog #: AF2309
1 Citation 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2309
AF2309-SP

All Smad8 Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Smad8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Smad8 in direct ELISAs and Western blots. In direct ELISAs, approximately 90% cross-reactivity with recombinant (rh) Smad1 is observed, approximately 20% cross-reactivity with rhSmad4 is observed, and less than 1% cross-reactivity with rhSmad5, rhSmad7, and rhDNAK is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Smad8
Ala158-Ser233
Accession # NP_005896

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Smad8

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human breast

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad8

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Smad8 was originally described as MADH6 and is also known as Smad9 and MADH9. Upon signaling by some BMP family members, Smad8 is phosphorylated resulting in its association with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Long Name

Mothers Against DPP Homolog 8

Entrez Gene IDs

4093 (Human)

Alternate Names

MADH6;MADH9;Mothers against decapentaplegic homolog;Mothers against decapentaplegic homolog 9;PPH2;SMAD8;SMAD8/9;SMAD8A;SMAD8B;SMAD9; Smad8; Smad9

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Smad8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Quantitative kinetics analysis of BMP2 uptake into cells and its modulation by BMP antagonists.
Authors: Alborzinia H, Schmidt-Glenewinkel H, Ilkavets I, Breitkopf-Heinlein K, Cheng X, Hortschansky P, Dooley S, Wolfl S
J Cell Sci, 2012-10-17;126(0):117-27.
Species: Human
Sample Types: Protein
Applications: Western Blot