Human SMARCA5/SNF2H Antibody

Catalog #: AF6825 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6825
AF6825-SP

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Human SMARCA5/SNF2H Antibody Summary

Species Reactivity

Human

Specificity

Detects human SMARCA5/SNF2H in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SMARCA1 and rhSMARC4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SAMRCA5/SNF2H
Asp77-Val185
Accession # O60264

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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SMARCA5/SNF2H in Human Spleen. SMARCA5/SNF2H was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human SMARCA5/SNF2H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6825) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMARCA5/SNF2H

SMARCA5 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 5; also SNF2H) is a 130-135 kDa member of the SNF2/RAD54 helicase family of molecules. It is a ubiquitously expressed, chromatin remodeling ATPase that increases the accessibility of nucleosomal DNA to DNA-binding proteins. It apparently does so by sliding histone octamers over DNA. SMARCA5 is a component of four chromatin-remodeling complexes, including CHRAC, RSF, ACF and WICH. Human SMARCA5 is 1052 amino acids (aa) in length. It contains an N-terminal helicase domain (aa 192-357), a helicase superfamily C-terminal domain (aa 474-605), and two SANT DNA-binding domains (aa 840-892 and 943-1007). Over aa 77-185, human SMARCA5 shares 99% aa identity with mouse SMARCA5.

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Entrez Gene IDs

8467 (Human); 93762 (Mouse); 307766 (Rat)

Alternate Names

EC 3.6.1; EC 3.6.4.-; hISWI; hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; ISWI; SMARCA5; SNF2H; SNF2Hsucrose nonfermenting-like 5; subfamily a, member 5; Sucrose nonfermenting protein 2 homolog; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5; WCRF135