Human SMPD1 Antibody Summary
His62-Pro628
Accession # NP_000534
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SMPD1 by Western Blot. Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMPD1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Human SMPD1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for SMPD1 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMPD1
Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.
- Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
- Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
- Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
- Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.
Product Datasheets
Citations for Human SMPD1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma
Authors: Gregory C. Wilson, Sameer H. Patel, Jiang Wang, Kui Xu, Kevin M. Turner, Katrin Anne Becker et al.
Journal of Molecular Medicine
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Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins
Authors: Xiahui Xiong, Chia-Fang Lee, Wenjing Li, Jiekai Yu, Linyu Zhu, Yongsoon Kim et al.
Biology Open
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Ceramide-induced cleavage of GPR64 intracellular domain drives Ewing sarcoma
Authors: Suvarna, K;Jayabal, P;Ma, X;Wang, H;Chen, Y;Weintraub, ST;Han, X;Houghton, PJ;Shiio, Y;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
Authors: O Ilnytska, K Lai, K Gorshkov, ML Schultz, BN Tran, M Jeziorek, TJ Kunkel, RD Azaria, HS McLoughlin, M Waghalter, Y Xu, M Schlame, N Altan-Bonn, W Zheng, AP Lieberman, R Dobrowolsk, J Storch
The Journal of Biological Chemistry, 2021-05-21;0(0):100813.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles
Authors: Ayan Mohamud Yusuf, Nina Hagemann, Xiaoni Zhang, Maria Zafar, Tanja Hussner, Carolin Bromkamp et al.
Basic Research in Cardiology
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