Human SOCS-2 Antibody

Catalog #: MAB4979 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4979
MAB4979-SP

Detection of Human SOCS‑2 by Western Blot.

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All SOCS-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOCS-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOCS-2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 517206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SOCS-2
Met1-Gln197 (predicted)
Accession # O14508

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SOCS‑2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human SOCS-2 Monoclonal Antibody (Catalog # MAB4979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for SOCS-2 was detected at approximately 23-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-2

SOCS-2 (Suppressor of cytokine signaling 2; also CIS-2 and SSI-2) is a 25 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by multiple cell types. It blocks receptor signaling by binding to phosphorylated tyrosines in the cytoplasmic domain of select receptors, including the receptors for leptin, IGF-I and GH. Human SOCS-2 is 198 amino acids (aa) in length. It contains one SH2 domain (aa 48‑156) and a SOCS box (aa 160‑198) that mediates interaction with elongin B or C of the E3 ubiquitin ligase complex. There is one splice variant that shows an Arg-rich eight aa substitution for aa 31‑39. Full-length SOCS-2 is 94% and 96% aa identical to mouse and porcine SOCS-2, respectively.

Long Name

Suppressor Of Cytokine Signaling 2

Entrez Gene IDs

8835 (Human); 84607 (Rat)

Alternate Names

CIS2; CIS2CIS-2; Cish2; Cytokine-inducible SH2 protein 2; SOCS2; SOCS-2; SOCS-2STAT-induced STAT inhibitor 2; SSI2; SSI-2STAT induced STAT inhibitor-2; SSI2STAT-induced STAT inhibitor-2; STATI2; STATI2suppressor of cytokine signaling-2; suppressor of cytokine signaling 2