Human SOX3 Antibody

SOX3 in NTera‑2 Human Cell Line. SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SOX3 in U‑251 MG cells (positive) and HDLM‑2 cells (negative). SOX3 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SOX3 by Western Blot Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala <-> WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOX3 by Immunohistochemistry Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala <-> WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOX3 by Western Blot SOX3-26ala from mouse and human retains transactivation activity.A) COS-7 cells were transfected with pcDNA3.1 expression vector containing either mouse Sox3, human SOX3, mouse Sox3-26ala, human SOX3-26ala or an empty vector control. Values represent mean normalised luciferase values plus standard deviation of four independent experiments measured 48 hours after transfection. Student's T-tests (two tailed, unequal variance) of SOX3-26ala from human or mouse compared to empty vector control show a statistically significant increase in luciferase activity. B) Nuclear protein lysates prepared from duplicate plates 48 hours after transfection show that less SOX3 is detected in the nucleus of cells expressing both mouse and human SOX3-26ala. pcDNA3.1-EGFP transfected cells were used as a control and prepared for both nuclear protein and whole cell extracts (WCE). Blotting for Histone H3, indicates equal loading and blotting for alpha -Tubulin shows an absence of cytoplasmic contamination in nuclear preparations. Transfection efficiency was determined by co-transfecting EGFP and counting positive cells prior to harvesting and found to be equal for all plasmids. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOX3 by Immunohistochemistry Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala <-> WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOX3 by Immunocytochemistry/ Immunofluorescence Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala <-> WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.