Human SPINK1 Antibody

Catalog #: MAB74961 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB74961-100
MAB74961-SP

Neutralization of SPINK1 Activity by Human SPINK1 Antibody.

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Human SPINK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SPINK1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 839304

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SPINK1
Asp24-Cys79
Accession # P00995

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Recombinant Human SPINK1 (50 ng/mL, Catalog # 7496-PI) inhibition of Trypsin (12.5 ng/mL) cleavage of the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 ( Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 0.8 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Neutralization of SPINK1 Activity by Human SPINK1 Antibody. Trypsin (12.5 ng/mL) activity is measured in the presence of Recombinant Human SPINK1 (50 ng/mL, 7496-PI) that has been preincubated with increasing concentration of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB74961). The ND₅₀ is approximately 0.8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPINK1

SPINK1 (Serine Protease Inhibitor Kazal-type 1; also TATI and PST1) is a 6-7 kDa secreted polypeptide initially identified as a tumor-derived trypsin inhibitor. It is widely expressed, and found in cells diverse as pancreatic acinar cells, columnar cells of the stomach, renal collecting duct epithelium, and ureteric transitional plus breast epithelium. SPINK1 is known to be secreted with pancreatic zymogens, and apparently inactivates prematurely-activated trypsin, thus protecting the pancreas from trypsin-mediated enzyme activation. It also is reported to regulate cell migration and proliferation, the latter effect attributed to its structural resemblance to EGF, and its ability to bind to activate the EGFR. Mature human SPINK1 is 56 amino acids (aa) in length (aa 24-79). It contains one Kazal-like domain (aa 26-79) that possesses a potential proteolytic cleavage site between Lys41-Ile42. An 11-12 kDa form in SDS-PAGE has been reported for SPINK1, possibly reflecting dimerization. Mature human SPINK1 shares 66% aa sequence identity with mouse Spink3, the mouse equivalent to human SPINK1.

Long Name

Serine Peptidase Inhibitor, Kazal Type 1

Entrez Gene IDs

6690 (Human); 20730 (Mouse); 266602 (Rat)

Alternate Names

pancreatic secretory trypsin inhibitor; PCTT; PCTTSpink3; PSTI; PSTISerine protease inhibitor Kazal-type 1; serine peptidase inhibitor, Kazal type 1; serine protease inhibitor, Kazal type 1; SPINK1; Spink3; TATI; TATITumor-associated trypsin inhibitor