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Human TFPI Antibody

Catalog #: AF2974
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2974
AF2974-SP
Detection of Human TFPI by Western Blot.
2 Images
Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFPI in direct ELISAs and Western blots. In directs ELISAs, less than 10% cross‑reactivity with recombinant mouse (rm) TFPI is observed and less than 1% cross-reactivity with recombinant human TFPI-2, and rmTFPI-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TFPI
Asp29-Lys282
Accession # P10646
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human TFPI (Catalog # 2974-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TFPI antibody by Western Blot. View Larger

Detection of Human TFPI by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFPI at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human TFPI antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TFPI by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TFPI at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA).

References
  1. Bai, H. et al. (2005) Thromb Haemost. 93:1055.
  2. Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
  3. Mine, S. et al. (2002) Biochemistry 41:78.
Long Name
Tissue Factor Pathway Inhibitor
Entrez Gene IDs
7035 (Human); 21788 (Mouse)
Alternate Names
convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor

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Citations for Human TFPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist-induced platelet functions reducing atherosclerosis and thrombosis phenotypes
    Authors: A Al Subayyi, YS Basmaeil, R Alenzi, T Khatlani
    Journal of Cellular and Molecular Medicine, 2021-09-18;0(0):.
  2. TMEM16E regulates endothelial cell procoagulant activity and thrombosis
    Authors: Alec A. Schmaier, Papa F. Anderson, Siyu M. Chen, Emale El-Darzi, Ivan Aivasovsky, Milan P. Kaushik et al.
    Journal of Clinical Investigation
  3. Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
    Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
    PloS Pathogens, 2022-01-31;18(1):e1010280.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: ELISA Detection, Western Blot
  4. The Impact of Anticoagulant Activity of Tissue Factor Pathway Inhibitor Measured by a Novel Functional Assay for Predicting Deep Venous Thrombosis in Trauma Patients: A Prospective Nested Case-Control Study
    Authors: Xiangyu Cao, Yu Su, Wenjie Zhang, Huiru Zhao, Meng Wen, Shan Lu et al.
    Clin Appl Thromb Hemost
  5. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
    Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
    Cell Rep, 2019-09-24;28(13):3329-3337.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies
    Authors: DA Slatter, CL Percy, K Allen-Redp, JM Gajsiewicz, NJ Brooks, A Clayton, VJ Tyrrell, M Rosas, SN Lauder, A Watson, M Dul, Y Garcia-Dia, M Aldrovandi, M Heurich, J Hall, JH Morrissey, S Lacroix-De, S Delignat, PV Jenkins, PW Collins, VB O'Donnell
    JCI Insight, 2018-03-22;3(6):.
    Species: Human
    Sample Types: Plasma
    Applications: Neutralization
  7. Correcting thrombin generation ex vivo using different haemostatic agents following cardiac surgery requiring the use of cardiopulmonary bypass
    Authors: Charles L. Percy, Rudolf Hartmann, Rhidian M. Jones, Subramaniam Balachandran, Dheeraj Mehta, Michael Dockal et al.
    Blood Coagulation & Fibrinolysis
  8. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
    Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
    Mol. Cell Proteomics, 2008-04-03;7(10):1974-82.
    Species: Human
    Sample Types: Serum
    Applications: Mass Spectrometry

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