Human TMPRSS9 Antibody

Catalog #: AF5688 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5688
AF5688-SP

Detection of Human TMPRSS9 by Western Blot.

1 Image

All TMPRSS9 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TMPRSS9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMPRSS9 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TMPRSS9
Glu190-Gln490
Accession # Q7Z410

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human TMPRSS9 by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human TMPRSS9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TMPRSS9 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMPRSS9

TMPRSS9 (Transmembrane protease, Serine 9; also Polyserine protease 1) is a 116-120 kDa (predicted) member of the TTSP family of membrane-bound serine proteases. It is expressed in fetal tissue, and converts pro-uPA into its active form. Human TMPRSS9 is a 1059 type II transmembrane (TM) protein. It has a 29 aa N-terminal cytoplasmic region and a 1008 aa extracellular domain (ECD) (aa 51-1059). The ECD contains one LDLR region (aa 153-190), followed by three Peptidase S1 domains (aa 203-436, 504-736, and 827-1058), the first two of which are catalytically active. Proteolytic cleavage can generate a soluble 34 kDa Serase 1 (aa 203-503), a 35 kDa Serase 2 (aa 504-826) and an inactive 25 kDa Serase 3 (aa 827-1059). Alternatively, truncated, TM forms of TMPRSS9 such as a 56 kDa TM isotype containing only the Serase 1 domain, or a 91 kDa (predicted) form containing the Serase 1, 2 and 3 domains may exist. Two potential splice forms are reported. One shows an insertion of 34 aa after Lys90 accompanied by a seven aa substitution for aa 491-1059. A second isoform shows a 34 aa substitution for aa 822-1059. Over aa 190-490, human TMPRSS9 shares 79% aa identity with mouse TMPRSS9.

Long Name

Transmembrane protease, serine 9

Entrez Gene IDs

360200 (Human); 432478 (Mouse); 314636 (Rat)

Alternate Names

EC 3.4.21; EC 3.4.21.4; Polyserase 1; Polyserase-1; Polyserase-I; Polyserine Protease 1; TMPRSS9; Transmembrane Protease Serine 9; Transmembrane Protease, Serine 9; Transmembrane Serine Protease 9