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Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

Catalog #: MAB4770
13 Citations 1 Review Datasheet / COA / SDS
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MAB4770
MAB4770-SP
Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry
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Product Details
Citations (13)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA‑1‑60(R).
Source
Monoclonal Mouse IgM Clone # TRA-1-60
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
2102Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
BG01V human embryonic stem cells and iPSC cells
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRA-1-60 antibody in BG01V Human Cells antibody by Flow Cytometry View Larger

Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (F0116).

Immunocytochemistry TRA-1-60 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

TRA‑1‑60 in BG01V Human Stem Cells. TRA-1-60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry TRA-1-60(R) antibody in ADLF1 and FAB2 Stem Cell Lines by Immunocytochemistry (ICC). View Larger

TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines. TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Flow Cytometry View Larger

Detection of TRA‑1‑60(R) in iPSC cells by Flow Cytometry. iPSC cells were stained with Mouse Anti-Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody Mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Canine TRA-1-60(R) by Flow Cytometry View Larger

Detection of Canine TRA-1-60(R) by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine TRA-1-60(R) by Flow Cytometry View Larger

Detection of Canine TRA-1-60(R) by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry View Larger

Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry View Larger

Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

References
  1. Andrews, P. et al. (1984) Hybridoma 3:347.
  2. Thomson, J. et al. (1998) Science 282:1145.
  3. Giwercman, A. et al. (1993) Cancer 72:1308.
  4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
  5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
  6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Entrez Gene IDs
5420 (Human)
Alternate Names
GCTM-2 antigen; GP135; gp200; PC; PCLP; PCLP-1; PDX; Podocalyxin; Podocalyxin-like protein 1; PODXL1; TRA160(R); TRA-1-60(R)

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Citations for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction
    Authors: Korover N, Etzion S, Cherniak A et al.
    Biological research
  2. Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
    Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
    Stem Cell Research, 2021-10-22;57(0):102583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  3. Induced pluripotent stem cells from subjects with Lesch-Nyhan disease
    Authors: DJ Sutcliffe, AR Dinasarapu, JE Visser, JD Hoed, F Seifar, P Joshi, I Ceballos-P, T Sardar, EJ Hess, YV Sun, Z Wen, ME Zwick, HA Jinnah
    Scientific Reports, 2021-04-19;11(1):8523.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines
    Authors: SJ Walker, AL Wagoner, D Leavitt, DL Mack
    Heliyon, 2021-04-03;7(4):e06617.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies
    Authors: Y Nagai, H Nakao, A Kojima, Y Komatsubar, Y Ohta, N Kawasaki, N Kawasaki, H Toyoda, T Kawasaki
    Biomolecules, 2021-03-29;11(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Transcriptional signatures of participant-derived neural progenitor cells and neurons implicate altered Wnt signaling in Phelan-McDermid syndrome and autism
    Authors: MS Breen, A Browne, GE Hoffman, S Stathopoul, K Brennand, JD Buxbaum, E Drapeau
    Mol Autism, 2020-06-19;11(1):53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Labeling
  7. The Role of FGF9 in the Production of Neural Retina and RPE in a Pluripotent Stem Cell Model of Early Human Retinal Development
    Authors: David M. Gamm, Eric Clark, Elizabeth E. Capowski, Ruchira Singh
    American Journal of Ophthalmology
  8. Generation of three induced pluripotent stem cell lines from postmortem tissue derived following sudden death of a young patient with STXBP1 mutation
    Authors: T Yamamoto, M Otsu, T Okumura, Y Horie, Y Ueno, H Taniguchi, M Ohtaka, M Nakanishi, Y Abe, T Murase, T Umehara, K Ikematsu
    Stem Cell Res, 2019-06-18;39(0):101485.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  9. Generation of kisspeptin-responsive GnRH neurons from human pluripotent stem cells
    Authors: Ariel Poliandri, Duncan Miller, Sasha Howard, Muriel Nobles, Gerard Ruiz-Babot, Stephen Harmer et al.
    Molecular and Cellular Endocrinology
  10. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017-03-07;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012-08-31;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Blood-Derived Human iPS Cells Generate Optic Vesicle–Like Structures with the Capacity to Form Retinal Laminae and Develop Synapses
    Authors: M. Joseph Phillips, Kyle A. Wallace, Sarah J. Dickerson, Michael J. Miller, Amelia D. Verhoeven, Jessica M. Martin et al.
    Investigative Opthalmology & Visual Science
  13. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2011-08-29;21(8):1272-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
By Anonymous on 10/20/2017
Application: Flow Sample Tested: H9 human embryonic stem cells Species: Human

The cells were stained with 1 µg of TRA-1-60 antibody in 100 µl FACS buffer at 4C for 30 min. After washing, the cells were incubated with anti-IgM-AF488 antibody at 4C for 20 min.

Flow Cytometry Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody MAB4770