Human TRF2 Antibody

Catalog #: AF5635 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5635-SP	Ships in 5-7 Days	25 ug / $149
AF5635	In Stock	100 ug / $449

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Detection of Human TRF2 by Western Blot.

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All TRF-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human TRF2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRF2
Gln78-Thr238
Accession # Q15554

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRF2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human TRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5635) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRF2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRF-2

TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55‑60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. TRF2 has been found up regulated in several human cancers. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13‑30), a dimerization domain (aa 46‑112), an NLS (aa 329‑333), and a DNA binding HTH myb-type domain (aa 442‑499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239‑500. Over aa 78‑238, human TRF2 shares 96% aa identity with mouse TRF2.

Long Name

Telomeric Repeat Binding Factor 2

Entrez Gene IDs

7014 (Human); 21750 (Mouse); 361403 (Rat)

Alternate Names

Telomeric DNA-binding protein; telomeric repeat binding factor 2; Telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TERF2; TRBF2; TRF2; TRF-2; TTAGGG repeat binding factor 2; TTAGGG repeat-binding factor 2