Human XPV Antibody

Catalog #: AF3417 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3417-SP	Ships in 5-7 Days	25 ug / $149
AF3417	In Stock	100 ug / $349

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Bulk Orders

1 Image

All XPV Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human XPV Antibody Summary

Species Reactivity

Human

Specificity

Detects human XPV in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human XPV
Met1-Phe400
Accession # Q9Y253

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Customers also Viewed

Human/Mouse/Rat Histone H2AX Antibody

Cat # MAB3406

(2)

Citations (9)

Insulin antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

Human/Mouse/Bovine Insulin Antibody

Cat # MAB1417

Citations (25)

Human ACE-2 Alexa Fluor® 594-conjugated Antibody

Cat # FAB933T

Recombinant Human Neuregulin-2/NRG2 His-tag Protein, CF

Cat # 11355-NR

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human XPV by Western Blot. Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3417) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XPV

Xeroderma pigmentosum variant (XPV) is a form of XP caused by mutation in the DNA polymerase eta (POLH) gene. DNA polymerase eta catalyzes translesion DNA synthesis past pyrimidine dimers, a mutation caused by exposure to ultraviolet (UV) light. XPV patients are sensitive to UV light and show an increased incidence of skin cancer.

Long Name

Xeroderma Pigmentosum Variant Type Protein

Entrez Gene IDs

5429 (Human); 80905 (Mouse); 316235 (Rat)

Alternate Names

DNA polymerase eta; DNA Polymerase, eta; EC 2.7.7.7; POLH; polymerase (DNA directed), eta; RAD30 homolog A; RAD30; RAD30A; RAD30AFLJ16395; Xeroderma pigmentosum variant type protein; XPV; XP-V; XPVFLJ21978