Human ZAG Antibody

Catalog #: MAB4764 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4764
MAB4764-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZAG Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZAG in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 842025

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ZAG
Met1-Ser298
Accession # P25311

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ZAG by Western Blot. Western blot shows human plasma and lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAG at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human ZAG by Simple Western^TM. Simple Western lane view shows lysates of human prostate tissue, loaded at 0.2 mg/mL. A specific band was detected for ZAG at approximately 59 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZAG

ZAG (zinc-alpha ₂-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.

Long Name

Zinc alpha-2-Glycoprotein

Entrez Gene IDs

563 (Human); 12007 (Mouse); 25294 (Rat)

Alternate Names

alpha-2-glycoprotein 1, zinc; alpha-2-glycoprotein 1, zinc-binding; AZGP1; ZA2G; ZAG; ZAGzn-alpha-2-GP; zinc; zinc-alpha-2-glycoprotein; zn-alpha-2-glycoprotein; ZNGP1