Mouse Cathepsin F Antibody

Catalog # Availability Size / Price Qty
MAB2544-SP Ships in 5-7 Days25 ug / $149
MAB2544 In Stock500 ug / $559

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Mouse Cathepsin F Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin F in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant mouse Cathepsin B, C, L, or S is observed and approximately 5% and 25% cross-reactivity with recombinant human Cathepsin F under non-reducing conditions and reducing conditions, respectively, is observed.
Source
Monoclonal Rat IgG2B Clone # 192204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin F
Ser20-Asn462
Accession # Q9WUT4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cathepsin F
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin F, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin F

Cathepsin F is a lysosomal cysteine protease that participates in protein degradation and has been implicated in tumor progression.

Entrez Gene IDs
8722 (Human); 56464 (Mouse)
Alternate Names
Cathepsin F; CATSFEC 3.4.22.41; CTSF; EC 3.4.22

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