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Mouse Cathepsin X/Z/P Antibody

Catalog #: AF1033
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1033
AF1033-SP
Cathepsin X/Z/P in Mouse Splenocytes.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cathepsin X/Z/P Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin X/Z/P in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
Ala23-Val306
Accession # Q9WUU7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Recombinant Mouse Cathepsin X/Z/P (0.1 µg/mL, Catalog # 1033-CY) cleavage of the fluorogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH (10 µM, Catalog # ES005). The Neutralization Dose (ND50) is 8 - 73 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Cathepsin X/Z/P antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Cathepsin X/Z/P in Mouse Splenocytes. Cathepsin X/Z/P was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1033) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References
  1. Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
  2. Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
  3. Nagler and Menard (1998) FEBS Lett. 434:135.
  4. Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
  5. Pungercar, et al. (2000) Pflugers Arch. 439:R119.
  6. Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, et al. (2001) Biol. Chem. 382:839.
  8. Therrien, et al. (2001) Biochemistry 40:2702.
  9. Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, et al. (1996) J. Biol. Chem. 271:30181.
Entrez Gene IDs
1522 (Human); 64138 (Mouse)
Alternate Names
Cathepsin P; Cathepsin X/Z/P; Cathepsin Z; CTSX; CTSZ

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Citations for Mouse Cathepsin X/Z/P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Increased Immunoreactivity of Cathepsins in the Rat Esophagus under Chronic Acid Reflux Esophagitis
    Authors: Masayuki Suyama, Masato Koike, Daisuke Asaoka, Hiroki Mori, Masako Oguro, Takashi Ueno et al.
    Journal of Histochemistry & Cytochemistry
  2. Development of Activity-Based Probes for Cathepsin X
    Authors: Margot G. Paulick, Matthew Bogyo
    ACS Chemical Biology
  3. Rapid and Progressive Loss of Multiple Retinal Cell Types in Cathepsin D-Deficient Mice—An Animal Model of CLN10 Disease †
    Authors: Mahmoud Bassal, Junling Liu, Wanda Jankowiak, Paul Saftig, Udo Bartsch
    Cells
  4. Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly
    Authors: E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm
    Nature Communications, 2022-08-17;13(1):4848.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Mice deficient in the lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) display a complex retinal phenotype
    Authors: Y Atiskova, S Bartsch, T Danyukova, E Becker, C Hagel, S Storch, U Bartsch
    Sci Rep, 2019-10-02;9(1):14185.
    Species: Mouse
    Sample Types: Tissue Culture Supernates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  6. Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers
    Authors: DM Chopyk, P Kumar, R Raeman, Y Liu, T Smith, FA Anania
    Physiol Rep, 2017-12-01;5(23):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  7. Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation
    Authors: Janko Kos
    Neuropharmacology, 2016-11-23;114(0):88-100.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  8. Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
    Authors: Makrypidi G, Damme M, Muller-Loennies S, Trusch M, Schmidt B, Schluter H, Heeren J, Lubke T, Saftig P, Braulke T
    Mol. Cell. Biol., 2011-12-12;32(4):774-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011-12-01;25(23):2465-79.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin.
    Authors: Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D
    Blood, 2011-07-27;118(12):3384-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009-07-17;110(6):1931-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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