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Mouse CCL25/TECK Antibody

Catalog #: MAB4811
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4811
MAB4811-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL25/TECK Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL25/TECK in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, and recombinant rat CCL20 is observed.
Source
Monoclonal Rat IgG2A Clone # 89827
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL25/TECK
Gln24-Asn144
Accession # O35903.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL25/TECK (Catalog # 481-TK)
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse intestine and perfusion fixed frozen sections of rat intestine

Mouse CCL25/TECK Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL25/TECK Biotinylated Antibody (Catalog # BAF481)

Standard: Recombinant Mouse CCL25/TECK Protein (Catalog # 481-TK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL25/TECK

CCL25 (thymus-expressed chemokine), also known as TECK (thymus-expressed chemokine), is a CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein.  Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. CCL25 functions through interactions with CCR9 and is chemotactic for activated macrophages, dendritic cells and thymocytes (1).

References
  1. Vicari, A.P. et al. (1997) Immunity 7:291.
Entrez Gene IDs
6370 (Human); 20300 (Mouse)
Alternate Names
C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine

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Citations for Mouse CCL25/TECK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: Pleural Lavage Fluid
    Applications: ELISA Development
  2. Functional characterization of the CCL25 promoter in small intestinal epithelial cells suggests a regulatory role for caudal-related homeobox (Cdx) transcription factors.
    Authors: Ericsson A, Kotarsky K, Svensson M, Sigvardsson M, Agace W
    J. Immunol., 2006-03-15;176(6):3642-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Thymic T cell development and progenitor localization depend on CCR7.
    Authors: Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Forster R
    J. Exp. Med., 2004-08-09;200(4):481-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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