Mouse CCL3/MIP-1 alpha Antibody Summary
Ala24-Ala92
Accession # Q5QNW0
Applications
Mouse CCL3/MIP-1 alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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CCL3/MIP‑1 alpha in Mouse Thymus. CCL3/MIP-1a was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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CCL3/MIP‑1 alpha in RAW 264.7 Mouse Cell Line. CCL3/MIP-1a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/MIP‑1 alpha Antibody. Recombinant Mouse CCL3/MIP-1a (Catalog # 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/ MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA). The ND50 is typically 0.05-0.2 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.
Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
Product Datasheets
Citations for Mouse CCL3/MIP-1 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Novel Therapeutic Targeting of CCL3-CCR4 Axis Mediated Apoptotic Intesitnal Injury in Necrotizing Enterocolitis
Authors: Xi Yuan, Zihan Xiong, Wei Liu, Yue Li, Hongdong Li, Xuemei Zhang et al.
Frontiers in Immunology
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Timing, initiation and function: An in-depth exploration of the interaction network among neutrophil extracellular traps related genes in acute pancreatitis
Authors: Zhang, H;Wang, Z;Li, J;Jia, Y;Li, F;
International immunopharmacology
Species: Mouse
Sample Types: In Vivo
Applications: In vivo assay -
Pharmacological Modulation of the MIP-1 Family and Their Receptors Reduces Neuropathic Pain Symptoms and Influences Morphine Analgesia: Evidence from a Mouse Model
Authors: Ciechanowska, A;Pawlik, K;Ciapa?a, K;Mika, J;
Brain sciences
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity
Authors: P Bhattachar, N Ismail, A Saxena, S Gannavaram, R Dey, T Oljuskin, A Akue, K Takeda, J Yu, S Karmakar, PK Dagur, JP McCoy, HL Nakhasi
PloS Neglected Tropical Diseases, 2022-02-22;16(2):e0010224.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile Brain
Authors: AM Osman, Y Sun, TC Burns, L He, N Kee, N Oliva-Vila, A Alevyzaki, K Zhou, L Louhivuori, P Uhlén, E Hedlund, C Betsholtz, VM Lauschke, J Kele, K Blomgren
Cell Rep, 2020-06-02;31(9):107699.
Species: Mouse
Sample Types:
Applications: IHC -
Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
Oncogene, 2019-02-14;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation
Authors: W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing
Nat Commun, 2018-12-03;9(1):5127.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
Sci Rep, 2017-04-20;7(0):46514.
Species: Mouse, Rat
Sample Types: Whole Cells
Applications: Neutralization -
Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy
Authors: David Blum
Brain, 2016-11-05;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development -
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
Am. J. Pathol., 2011-10-01;179(6):2730-9.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development -
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010-02-08;120(3):907-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Recruitment and differentiation of conventional dendritic cell precursors in tumors.
Authors: Diao J, Zhao J, Winter E, Cattral MS
J. Immunol., 2009-12-21;184(3):1261-7.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009-10-07;183(9):5799-806.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
J. Biol. Chem., 2009-07-10;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
J. Immunol., 2008-11-01;181(9):6384-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
J. Exp. Med., 2008-08-11;205(9):2075-84.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
Eur. J. Immunol., 2006-08-01;36(8):2025-34.
Species: Mouse
Sample Types: Tissue Secretion
Applications: ELISA Development -
Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2005-02-26;231(1):103-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
Brain, 2005-02-02;128(0):854-66.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Authors: Cardona AE, Gonzalez PA, Teale JM
Infect. Immun., 2003-05-01;71(5):2634-42.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Authors: Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA
J. Immunol., 2001-08-01;167(3):1584-91.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISpot Development -
Gestational Bisphenol A Exposure Impacts Embryonic Hypothalamic Microglia Numbers, Ramification, and Phagocytic Cups
Authors: Jessica M. Rosin, Nikol Tretiakov, Emily Hanniman, Kiana Hampton, Deborah M. Kurrasch
Frontiers in Neuroscience
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Neuroectodermal Stem Cells Grafted into the Injured Spinal Cord Induce Both Axonal Regeneration and Morphological Restoration via Multiple Mechanisms
Authors: Krisztián Pajer, Tamás Bellák, Heinz Redl, Antal Nógrádi
Journal of Neurotrauma
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The role of CCR1 and therapeutic effects of anti‐CCL3 antibody in herpes simplex virus‐induced Behçet's disease mouse model
Authors: Hasan M. Sayeed, Eun‐So Lee, Hae‐Ok Byun, Seonghyang Sohn
Immunology
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Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die
Authors: Krisztián Pajer, Georg A. Feichtinger, Gábor Márton, Sonja Sabitzer, Dieter Klein, Heinz Redl et al.
Experimental Neurology
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Cytotoxic T cells swarm by homotypic chemokine signalling
Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
eLife
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IL9 Polarizes Macrophages to M1 and Induces the Infiltration of Antitumor Immune Cells via MIP-1 and CXCR3 Chemokines
Authors: Van Anh Do-Thi, Sang Min Park, Song Mi Park, Hye Jin Jeong, Geunyoung Cho, Hyun-Jung An et al.
Cancer Research Communications
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This antibody was paired with BAF450 to build a sandwich ELISA to measure MIP-1a in mouse plasma.
