Mouse CCL6/C10 Antibody

Catalog #: AB-487-NA Datasheet / COA / SDS

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AB-487-NA

Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody.

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All CCL6/C10 Antibodies

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Mouse CCL6/C10 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL6/C10 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse CCL6/C10
Gly22-Ala116
Accession # P27784

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CCL6/C10 (Catalog # 487-C)

Neutralization

Measured by its ability to neutralize CCL6/C10-induced chemotaxis in human monocytes. The Neutralization Dose (ND₅₀) is typically 70-100 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CCL6/C10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL6/C10 Polyclonal Antibody (Catalog # AB-487-NA). The ND₅₀ is typically 70-100 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL6/C10

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.

Entrez Gene IDs

20305 (Mouse); 287910 (Rat)

Alternate Names

C10; c10;C-C motif chemokine 6;MRP;MRP-1;Scy;Scya6; CCL6; MRP-1; SCYA6

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