Mouse CCR5 Antibody

Catalog #: MAB18041 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB18041-100
MAB18041-SP

Detection of CCR5 in HEK293 Human Cell Line Transfected with Mouse CCR5 and eGFP by Flow Cytometry.

1 Image

All CCR5 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR5 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR5 transfectants but not the parental cell line in Flow Cytometry and Western blots.

Source

Recombinant Monoclonal Rat IgG_2B Clone # 225307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

C6 rat glioma cell line transfected with mouse CCR5
Accession # P51682

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CCR5 in HEK293 Human Cell Line Transfected with Mouse CCR5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse CCR5 and eGFP was stained with (A) Rat Anti-Mouse CCR5 Monoclonal Antibody (Catalog # MAB18041) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by PE-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 (CC chemokine receptor 5; also CD195 and MIP-1a receptor) is a 41 - 44 kDa member of the G-protein coupled receptor #1 family of proteins. It is expressed on Treg cells, NK cells, neurons, macrophages and Th1 cells. CCR5 mediates cell adhesion and migration induced by several chemokines including CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, and CCL8/MCP-2. It also functions as a coreceptor for macrophage-tropic HIV-1 infection. CCR5 contains an O-glycosylated and sulfated extracellular N-terminus (aa 1 - 32), and a phosphorylated and palmitoylated intracellular C-terminus (aa 304 - 354). CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Within aa 1 - 32, mouse CCR5 shares 72% and 91% amino acid sequence identity with human and rat CCR5, respectively.

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Alternate Names

C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5